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90718

Sigma-Aldrich

Fluorescein isothiocyanate–dextran

average Mw 70,000

Synonym(s):
FITC–Dextran 70, FITC–Dextran
CAS Number:
MDL number:
NACRES:
NA.32

biological source

synthetic

Quality Level

conjugate

FITC conjugate

form

powder or crystals

mol wt

average Mw 70,000

extent of labeling

0.001-0.020 mol/mol FITC glucose

fluorescence

λex 493 nm; λem 520 nm±5 nm in 0.1 M Tris pH 8.0

application(s)

cell analysis

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This Item
6965846945FD2000S
form

powder or crystals

form

powder or crystals

form

powder

form

powder

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

mol wt

average Mw 70,000

mol wt

average mol wt 150,000

mol wt

average mol wt 70,000

mol wt

average mol wt 2,000,000

extent of labeling

0.001-0.020 mol/mol FITC glucose

extent of labeling

0.001-0.020 mol/mol FITC glucose

extent of labeling

~0.004 mol/mol FITC glucose

extent of labeling

0.003-0.020 mol FITC per mol glucose

fluorescence

λex 493 nm; λem 520 nm±5 nm in 0.1 M Tris pH 8.0

fluorescence

λex 493 nm; λem 520 nm±5 nm in 0.1 M Tris pH 8.0

fluorescence

λex 492 nm; λem 518 nm in ethanol

fluorescence

-

General description

Fluorescein isothiocyanate–dextran is an anionic fluorescent probe. The carboxyl group provides the negative charge, which improves the overall permeability of the molecule. It is supplied as a yellow powder that is freely soluble in water and electrolyte solutions. Fluorescein isothiocyanate–dextran is insoluble in most organic samples such as ethanol, methanol, acetone, and chloroform., however, it dissolves in DMSO, formamide, and a few other polar organic solvents.

Application

Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.
Fluorescein isothiocyanate–dextran has an application of cell analysis, Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals. FITC-dextrans are primary source of studying permeability and transport in cells and tissues and can also be a tool for permeability studies of other materials.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Jun-Ichi Takino et al.
Scientific reports, 11(1), 2959-2959 (2021-02-05)
Advanced glycation end-products (AGEs) are formed by the non-enzymatic reaction of sugars and proteins. Among the AGEs, glyceraldehyde-derived toxic AGEs (TAGE) are associated with various diseases, including diabetic complications such as diabetic retinopathy (DR). The risk of developing DR is
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Journal of virology, 82(22), 11273-11282 (2008-09-05)
In order to analyze whether measles virus (MV) is transported via transmigrating leukocytes across endothelial barriers or whether virus spreads via infection of endothelial cells and basolateral release, we investigated the migratory behavior of infected human primary T lymphocytes across
Gabor Eros et al.
Arthritis research & therapy, 11(2), R43-R43 (2009-03-20)
Phosphatidylcholine and phosphatidylcholine-derived metabolites exhibit anti-inflammatory properties in various stress conditions. We hypothesized that dietary phosphatidylcholine may potentially function as an anti-inflammatory substance and may decrease inflammatory activation in a chronic murine model of rheumatoid arthritis (collagen-induced arthritis). The experiments
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PloS one, 6(6), e21411-e21411 (2011-07-07)
VEGFR1 and 2 signaling have both been increasingly shown to mediate complications of ischemic retinopathies, including retinopathy of prematurity (ROP), age-related macular degeneration (AMD), and diabetic retinopathy (DR). This study evaluates the effects of blocking VEGFR1 and 2 on pathological

Articles

FITC-labelled polysaccharides

Additional information about FITC-labelled polysaccarides including FITC-Dextran, FITC-DEAE-dextran (FDD), FITC-carboxymethyl-dextran (FCM-Dextran). This page discusses the impact of weight-average molecular weight on the respective molecule dimensions and other physical properties

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