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S2890

Sigma-Aldrich

Streptavidin−Alkaline Phosphatase from Streptomyces avidinii

lyophilized powder, ≥700 DEA units/mg protein

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Synonym(s):
Streptavidin-AP, Streptavidin−AP from Streptomyces avidinii
MDL number:
MFCD00132384
NACRES:
NA.46

conjugate

alkaline phosphatase conjugate

Quality Level

200

form

lyophilized powder

specific activity

≥700 DEA units/mg protein

composition

Protein, ≥40% biuret

storage temp.

−20°C

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Sigma-Aldrich

Sigma-Aldrich

S2890

Streptavidin−Alkaline Phosphatase from Streptomyces avidinii

Streptavidin−FITC from Streptomyces avidinii essentially salt-free, lyophilized powder, ≥5 units/mg protein

Sigma-Aldrich

S3762

Streptavidin−FITC from Streptomyces avidinii

Streptavidin−Peroxidase from Streptomyces avidinii lyophilized powder

Sigma-Aldrich

S5512

Streptavidin−Peroxidase from Streptomyces avidinii

Sigma-Aldrich

Sigma-Aldrich

S6402

Streptavidin−Cy3™ from Streptomyces avidinii

specific activity

≥700 DEA units/mg protein

specific activity

≥5 units/mg protein

specific activity

-

specific activity

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

conjugate

alkaline phosphatase conjugate

conjugate

FITC conjugate

conjugate

peroxidase conjugate

conjugate

CY3 conjugate

composition

Protein, ≥40% biuret

composition

-

composition

Protein, ≥70% E1%/280

composition

-

General description

Streptavidin, a 58 kDa protein, is a homotetramer purified from Streptomyces avidinii. It has a high affinity for biotin. Streptavidin has been conjugated with either peroxidase or alkaline phosphatase (ALP). These enzymes are used as protein conjugates because of their comparative stability and a wide range of suitable substrates for different applications. ALP conjugates to streptavidin in immunoassays.

Application

Streptavidin-alkaline phosphatase from Streptomyces avidinii has been used in enzyme-linked immune absorbent spot (ELISpot) to detect interferon (IFN-γ) producing cells.
The optimal working dilution should be determined empirically using a range of dilutions from a 1 mg/ml stock in buffer.

Unit Definition

One alkaline phosphatase unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 °C.

Physical form

Lyophilized powder containing trehalose with EPPS, phosphate and traces of magnesium chloride and zinc chloride.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Effects of bovine Herpesvirus Type 5 on development of in vitro-produced bovine embryos.
Silva-Frade C
Theriogenology, 73(3), 324-331 (2010)
Sialoside specificity of the siglec family assessed using novel multivalent probes: identification of potent inhibitors of myelin-associated glycoprotein.
Blixt O et al
The Journal of Biological Chemistry, 278(33), 31007-31019 (2003)
Bystander activation of irrelevant CD4+ T cells following antigen-specific vaccination occurs in the presence and absence of adjuvant
van Aalst S, et al.
PLoS ONE, 12(5), e0177365-e0177365 (2017)
Ola Blixt et al.
The Journal of biological chemistry, 278(33), 31007-31019 (2003-05-30)
Ten of the 11 known human siglecs or their murine orthologs have been evaluated for their specificity for over 25 synthetic sialosides representing most of the major sequences terminating carbohydrate groups of glycoproteins and glycolipids. Analysis has been performed using
Microbial Resource Conservation: Conventional to Modern Approaches (2018)
View All Related Papers

Protocols

Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase (EC 3.1.3.1)

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

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