Merck
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A1227

Sigma-Aldrich

ABTS Enhancer

for peroxidase detection

Synonym(s):
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Enhancer
NACRES:
NA.83

Quality Level

form

liquid

storage temp.

2-8°C

Application

2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) has been used for the determination of laccase activity in Trametes versicolor. It may be suitable for use in enzyme-linked immunosorbent assay (ELISA) for detecting signal strength emitted by cells.

Biochem/physiol Actions

2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) is a non-phenolic substrate. It might exhibit antioxidant property. The detection of the horseradish peroxidase substrate ABTS can be improved by the addition of a chemical enhancer. The ABTS enhancer increases the absorbance of the oxidized product 50-150%. This increase is used to improve assay sensitivity or conserve assay components.

Physical form

Supplied as an 11X concentrate

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Aquatic Chronic 3 - Carc. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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