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G 418 disulfate salt

powder, BioReagent, suitable for cell culture

Antibiotic G418
Empirical Formula (Hill Notation):
C20H40N4O10 · 2H2SO4
CAS Number:
Molecular Weight:
MDL number:
PubChem Substance ID:

Quality Level

product line




optical activity

[α]/D (Specific Rotation: +104.4o (c=0.3% in H2O at 26oC))


≥720 μg per mg (Dried basis)

mol wt



pkg of 1 g
pkg of 25 g
pkg of 5 g


cell culture | mammalian: suitable


white to off-white


H2O: 100 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 8 days.)

Mode of action

protein synthesis | interferes

antibiotic activity spectrum

Gram-negative bacteria

storage temp.


SMILES string




InChI key


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General description

Chemical structure: aminoglycoside
G-418 is an aminoglycoside antibiotic, which is produced by Micromonospora rhodorangea NRRL 5326 strain. It has a broad range of antibacterial activity both in vivo and in vitro. It also shows activity against protozoa, cecal amoebiasis, and helminthes. It is commonly known as geneticin selective antibiotic.


G418 disulfate salt is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene†. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL.


1, 5, 25 g in glass bottle

Biochem/physiol Actions

G-418 inhibits protein synthesis at the early stages of elongation, post-initiation, at the initiation stage of translation as well as just before the initiation stage of translation.


The product is stable for two years when stored at 2-8°C. Stock solutions are stable at 37°C for 8 days.

Preparation Note

G418 powder is soluble in water at 100 mg/mL.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.


Health hazard



Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

13 - Non Combustible Solids

WGK Germany


Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

G H Wagman et al.
Antimicrobial agents and chemotherapy, 6(2), 144-149 (1974-08-01)
Antibiotic G-418 is a new aminoglycoside produced as the major component by a new species of Micromonospora, M. rhodorangea NRRL 5326. The antibiotic is prepared by submerged fermentation in a soybean-dextrin medium. Antibiotic G-418 is adsorbed on a cationic-exchange resin...
D C Eustice et al.
Antimicrobial agents and chemotherapy, 26(1), 53-60 (1984-07-01)
Tetrahymena thermophila is a eucaryotic organism that is highly susceptible to growth inhibition by aminoglycoside antibiotics. Concentrations of paromomycin, gentamicin G418, and hygromycin B at 22, 10, and 17 microM, respectively, inhibited growth by 50%. A combination of in vitro...
Serene R Lai et al.
Methods in molecular biology (Clifton, N.J.), 405, 31-37 (2008-03-29)
RNA interference (RNAi) is one of the most commonly used procedures for gene targeting in today's cutting edge technology and has great potential for use in clinical therapy. Using a plasmid construct that exogenously expresses short-hairpin RNAs (shRNAs) targeting a...
Hillary C Valley et al.
Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society, 18(4), 476-483 (2018-12-20)
Assessment of approved drugs and developmental drug candidates for rare cystic fibrosis (CF)-causing variants of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) requires abundant material from relevant models. Isogenic cell lines harboring CFTR variants in the native genomic context were...
Nikolay P Kandul et al.
Nature communications, 11(1), 2106-2106 (2020-05-02)
Here, we describe a drug-inducible genetic system for insect sex-separation that demonstrates proof-of-principle for positive sex selection in D. melanogaster. The system exploits the toxicity of commonly used broad-spectrum antibiotics geneticin and puromycin to kill the non-rescued sex. Sex-specific rescue...


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