Used to alkylate protein after reducing, prior to a trypsin digestion. Very easy to reconstitute, 1 vial covers many samples (I use at 15mM, reconstitute to 1.5M, the volume I use covers 200 samples). Works very quickly, 15 mins in the dark at room temp. Always shows as having worked in results.
A3221
Iodoacetamide
Iodoacetamide
1 of 2 reviewers received a sample product or took part in a promotion
Single use vial of 56 mg
Synonym(s):
2-Iodoacetamide, IAA, Monoiodoacetamide, alpha-Iodoacetamide
Sign Into View Organizational & Contract Pricing
All Photos(3)
Select a Size
Change View
10 VIALS
CHF 197.00
About This Item
Linear Formula:
ICH2CONH2
CAS Number:
Molecular Weight:
184.96
Beilstein:
1739080
EC Number:
MDL number:
UNSPSC Code:
12352111
PubChem Substance ID:
NACRES:
NA.56
Recommended Products
biological source
synthetic (organic)
Assay
≥99% (HPLC)
form
powder
packaging
vial of 56 mg (Single use)
storage condition
(Tightly closed. Dry. Keep in a well-ventilated place. Keep locked up or in an area accessible
only to qualified or authorized persons.)
color
white to off-white
mp
92-95 °C (lit.)
solubility
H2O: soluble 50 mg/mL, clear, colorless to faintly yellow
suitability
suitable for LC-MS
application(s)
microbiology
storage temp.
2-8°C
SMILES string
NC(=O)CI
InChI
1S/C2H4INO/c3-1-2(4)5/h1H2,(H2,4,5)
InChI key
PGLTVOMIXTUURA-UHFFFAOYSA-N
Looking for similar products? Visit Product Comparison Guide
1 of 4
This Item | I5161 | 407710 | I1149 |
|---|---|---|---|
| assay ≥99% (HPLC) | assay ≥99% (NMR) | assay ≥99% (HPLC) | assay ≥99% (NMR) |
| biological source synthetic (organic) | biological source synthetic | biological source - | biological source synthetic (organic) |
| Quality Level 200 | Quality Level 400 | Quality Level 200 | Quality Level 300 |
| form powder | form powder | form crystalline solid | form powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
| solubility H2O: soluble 50 mg/mL, clear, colorless to faintly yellow | solubility H2O: 0.5 M, clear, colorless | solubility methanol: 1:20, DMF: soluble, ethanol: soluble, water: soluble | solubility H2O: 0.5 M, clear, colorless |
General description
2-Iodoacetamide is a thiol-alkylating agent frequently used in peptide mapping for biochemical, cell culture and proteomics research. It functions similarly to iodoacetate, primarily binding covalently with the thiol group of cysteine in proteins, thereby preventing the formation of disulfide bonds. This attribute is crucial for several applications, including 2-D protein electrophoresis to reduce streaking and improve resolution, as an inhibitor of deubiquitinase enzymes (DUBs) and a peptidase inhibitor. It is often employed during sample preparation for de novo peptide sequencing with protein mass spectrometry.
It has specific interactions with cysteine and histidine residues in proteins, affecting enzymes like ribonuclease. While it may react slowly with histidine, its inhibitory properties contribute to the research on proteases, such as cysteine proteases. Iodoacetamide is known for its irreversibility in inhibiting enzymes and its ability to form stable protein derivatives, making it valuable in studying protein structures, protein behavior, and enzyme inhibition.
It has specific interactions with cysteine and histidine residues in proteins, affecting enzymes like ribonuclease. While it may react slowly with histidine, its inhibitory properties contribute to the research on proteases, such as cysteine proteases. Iodoacetamide is known for its irreversibility in inhibiting enzymes and its ability to form stable protein derivatives, making it valuable in studying protein structures, protein behavior, and enzyme inhibition.
Application
Biochem/physiol Actions
Iodoacetamide acts as an alkylating reagent for cysteine residues in peptide sequencing. It is an irreversible inhibitor of enzymes with cysteine at the active site. It reacts much more slowly with histidine residues, but that activity is responsible for inhibiting ribonuclease.
Features and Benefits
High quality compound suitable for multiple research applications
Packaging
Packaged in sealed ampules
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Legal Information
Technology developed in partnership with Proteome Systems™
Proteome Systems is a trademark of Proteome Systems Ltd
comparable product
Product No.
Description
Pricing
related product
Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Oral - Aquatic Chronic 4 - Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
