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A4418

dd_molecule_design

Molecular Biology, ≥99.0%

dd_molecule_design
1 of 2 reviewers received a sample product or took part in a promotion

Synonym(s):

Mascagnite, Ammonium sulfate

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100 g
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CHF 68.50
500 g
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CHF 199.00
1 kg
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CHF 349.00
5 kg
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CHF 1’360.00

About This Item

Linear Formula:
(NH4)2SO4
CAS Number:
Molecular Weight:
132.14
PubChem Substance ID:
eCl@ss:
38030408
UNSPSC Code:
12352314
NACRES:
NA.31
EC Number:
231-984-1
MDL number:
Assay:
≥99.0%
Grade:
Molecular Biology
Form:
powder
Solubility:
H2O: 0.667 g/mL

CHF 68.50


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grade

Molecular Biology

Quality Level

assay

≥99.0%

form

powder

technique(s)

affinity binding assay: suitable, protein purification: suitable

impurities

≤0.005% Insolubles

pH

5.0-6 (25 °C, 132 g/L)

mp

>280 °C (dec.) (lit.)

solubility

H2O: 0.667 g/mL

density

1.77 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤5 ppm

cation traces

Cu: ≤5 ppm, Fe: ≤5 ppm, Zn: ≤5 ppm, heavy metals (as Pb): ≤2 ppm

absorption

≤0.025 at 280 at 1 M, ≤0.03 at 260 at 1 M

suitability

suitable for flow cytometry, suitable for molecular biology

application(s)

advanced drug delivery
metabolomics
microbiology

foreign activity

DNase, RNase, and protease, none detected

SMILES string

N.N.OS(O)(=O)=O

InChI

1S/2H3N.H2O4S/c;;1-5(2,3)4/h2*1H3;(H2,1,2,3,4)

InChI key

BFNBIHQBYMNNAN-UHFFFAOYSA-N

General description

Ammonium sulfate is a versatile inorganic sulfate salt, plays crucial role in biochemical research, molecular biology, and cell culture. It serves as an indispensable tool for purifying antibodies and precipitating or fractionating proteins. Furthermore, it is instrumental in crystallographic analysis of nucleic acids and proteins and also used as a nitrogen supplement in synthetic laboratory media. Our premium-grade Ammonium Sulfate is meticulously crafted to be DNase, RNase, and Protease-free, ensuring its suitability for cutting-edge research.

Application

Ammonium sulfate helps in quick precipitation of celluar proteins. Ammonium sulfate may be used for the precipitation or fractionation of proteins or for purification of antibodies. It is also useful for crystallographic analysis of nucleic acids and proteins.Ammonium sulfate has been used
  • in the preparation of ′Blue Silver′ colloidal Coomassie stain for proteins. [1]
  • to precipitate the Shiga-like toxin from the culture supernatant.
  • for precipitaion of viral particles from the culture supernatant. [2]

Features and Benefits

  • Heavy metals (as Pb): ≤2 ppm
  • Free from DNase, RNase, and protease

Other Notes

For additional information on our range of Biochemicals, please complete this form.

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This Item
2150-OP1.0121131119
form

powder

form

crystalline powder

form

solid

form

crystals

assay

≥99.0%

assay

-

assay

-

assay

≥99%

solubility

H2O: 0.667 g/mL

solubility

-

solubility

754-764 g/L

solubility

-

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

200

grade

Molecular Biology

grade

reagent grade

grade

-

grade

ACS reagent, puriss. p.a.

pH

5.0-6 (25 °C, 132 g/L)

pH

-

pH

5 (100 g/L in H2O)

pH

5-6 (25 °C, 5%)


Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Related Content

Product Information Sheet


Assessment of Cell Cycle and Induction of Apoptosis by Shiga-like Toxin Produced by Escherichia coli O157:H7 in T47D Breast Cancer Cells Using Flow Cytometry
Wayan Suardana et al.
Asian Pacific Journal of Cancer Prevention, 23-10, 3247-3252 (2022)
Use of ammonium sulphate as a sulphur fertilizer: Implications for ammonia volatilization
David S Powlson, Chris J Dawson
Soil use and management, 38(1), 622-634 (2022)
Protein precipitation using ammonium sulfate
P Wingfield
Current Protocols in Protein Science / Editorial Board, John E. Coligan ... [Et Al.], 3 (2001)



Global Trade Item Number

SKUGTIN
A4418-1KG04061835542109
A4418-500G04061835542116
A4418-5KG04061833374474
A4418-100G04061835542093

Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. What is the average particle size (APS) of this powder?

    1 answer
    1. The particle size is not determined for this product.

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

Reviews

Active Filters

  1. New Jersey, USA
    • Review 1
    • Votes 3
    5 out of 5 stars.

    works well

    I used this product to perform ammonium sulfate-mediated precipitation on cell culture media, and it has worked very well. I will keep buying this in future.

    Helpful?

  2. 1 Ratings-only review