A5228
Anti-Actin, α-Smooth Muscle antibody, Mouse monoclonal
clone 1A4, purified from hybridoma cell culture
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
1A4, monoclonal
form
PBS solution
mol wt
antigen ~42 kDa
species reactivity
human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake
packaging
antibody small pack of 25 μL
technique(s)
immunocytochemistry: suitable using smooth muscle cells
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using smooth muscle cells
indirect ELISA: suitable
indirect immunofluorescence: 5-10 μg/mL using blood vessels in formalin-fixed, paraffin-embedded human tonsil or appendix tissue sections
microarray: suitable
western blot: suitable using smooth muscle cells
isotype
IgG2a
UniProt accession no.
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ACTA2(59)
mouse ... Acta2(11475)
rat ... Acta2(81633)
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General description
Immunogen
Application
- immunofluorescence staining
- immunohistochemistry
- immunoblotting
- immunocytochemistry
- enzyme linked immunosorbent assay (ELISA)
- western blotting
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
Biochem/physiol Actions
Physical form
Other Notes
Disclaimer
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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