Merck
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AP0100

Sigma-Aldrich

Alkaline Phosphatase Diethanolamine Activity Kit

sufficient for 100 tests

Synonym(s):
ALP Activity Kit
NACRES:
NA.26

usage

sufficient for 100 tests

Quality Level

storage temp.

2-8°C

General description

The Alkaline Phosphatase Diethanolamine Detection Kit provides ready-to-use reagents for detecting the presence of alkaline phosphatase activity. This simple assay to detect alkaline phosphatase activity uses p-Nitrophenyl Phosphate (pNPP) as the substrate.

Application

Alkaline Phosphatase Diethanolamine Activity Kit has been used:
  • in the detection of alkaline phosphatase (ALP) levels in the blood serum and osteoblasts[1]
  • for monitoring ALP levels during osteoblast differentiation in mouse myoblast C2C12 cells[2]
  • for determining ALP activity in the bone metastasis model of lung cancer[3]
  • for measuring ALP levels in colon tissue homogenates[4]

Suitability

Suitable for the measurement of alkaline phosphatase activity in a variety of samples

Principle

Alkaline phosphatase hydrolyzes pNPP to p-nitrophenol and inorganic phosphate. During incubation of the alkaline phosphatase sample and substrate at 37 C, the reaction is followed by monitoring the increase in absorbance at 405 nm.

Kit Components Also Available Separately

Product No.
Description
SDS

  • Alkaline Phosphatase Diethanolamine Reaction Buffer

  • P4744Phosphatase substrate, powder

  • P6774Phosphatase, Alkaline from bovine intestinal mucosa, buffered aqueous solution, ≥2,000 DEA units/mg protein

Pictograms

CorrosionHealth hazard

Signal Word

Danger

Hazard Classifications

Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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