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C0875

Sigma-Aldrich

Calcein

Powder

Synonym(s):

Bis[N,N−bis(carboxymethyl)aminomethyl]fluorescein, Fluorescein-bis(methyliminodiacetic acid), Fluorexon

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5 G
CHF 262.00
10 G
CHF 581.00
25 G
CHF 1’160.00

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5 G
CHF 262.00
10 G
CHF 581.00
25 G
CHF 1’160.00

About This Item

Empirical Formula (Hill Notation):
C30H26N2O13
CAS Number:
Molecular Weight:
622.53
Beilstein:
8181068
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

CHF 262.00


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Product Name

Calcein, Used for the fluorometric determination of calcium and EDTA titration of calcium in the presence of magnesium.

description

mixed isomers

form

powder

technique(s)

titration: suitable

color

yellow to brown

solubility

NaOH: 50 mg/mL

fluorescence

λex 470 nm; λem 509 nm (pH 7.4)

suitability

suitable for (Suitable for fluorometric determination of Ca Suitable for EDTA titration of Ca in the presence of Mg)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

OC(=O)CN(CC(O)=O)Cc1cc2c(Oc3cc(O)c(CN(CC(O)=O)CC(O)=O)cc3C24OC(=O)c5ccccc45)cc1O

InChI key

DEGAKNSWVGKMLS-UHFFFAOYSA-N

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This Item
M1255F6377M8010
Calcein Used for the fluorometric determination of calcium and EDTA titration of calcium in the presence of magnesium.

C0875

Calcein

suitability

suitable for (Suitable for fluorometric determination of Ca Suitable for EDTA titration of Ca in the presence of Mg)

suitability

suitable for indicator for EDTA titration

suitability

-

suitability

-

technique(s)

titration: suitable

technique(s)

titration: suitable

technique(s)

titration: suitable

technique(s)

-

color

yellow to brown

color

white to light yellow

color

orange to brown

color

-

fluorescence

λex 470 nm; λem 509 nm (pH 7.4)

fluorescence

λex 322 nm; λem 445 nm in methanol

fluorescence

λex 460 nm; λem 515 nm(lit.)

fluorescence

λex 319 nm; λem 384 nm (pH 9.0), λex 360 nm; λem 449 nm (Reaction product), λex 368 nm; λem 505 nm in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

form

powder

form

powder

form

powder

form

powder

Application

Calcein has been used:
  • in whole cell staining[1]
  • in histomorphometry and micro-computed tomography[2]
  • to label active bone-forming surfaces[3]

Biochem/physiol Actions

Calcein is a calcium-dependent fluorescent molecule. It is mainly required to study bone metabolism under in vivo conditions and helps in the staining of pit area under in vitro conditions.[4] Calcein is used for the fluorometric determination of calcium and EDTA (ethylenediaminetetraacetic acid) titration of calcium.[5]

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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