Merck
  • C8052
All Photos(1)

C8052

Sigma-Aldrich

Cholera Toxin from Vibrio cholerae

≥90% (SDS-PAGE), lyophilized powder

All Photos(1)
Synonym(s):
CTX, Cholera enterotoxin, Choleragen
CAS Number:
9012-63-9
MDL number:
MFCD00130776
NACRES:
NA.77

Quality Level

300

Assay

≥90% (SDS-PAGE)

form

lyophilized powder

composition

Protein, ~5% Lowry-TCA

solubility

H2O: 10 mg/mL

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

1 of 4

This Item
H2519H8891P7208
Sigma-Aldrich

Sigma-Aldrich

C8052

Cholera Toxin from Vibrio cholerae

Heparinase I from Flavobacterium heparinum Lyophilized powder stabilized with approx. 25% bovine serum albumin, ≥200 units/mg protein (enzyme + BSA)

Sigma-Aldrich

H2519

Heparinase I from Flavobacterium heparinum

Heparinase III from Flavobacterium heparinum Lyophilized powder stabilized with approx. 25% (w/w) bovine serum albumin, ≥30 units/mg protein (enzyme + BSA)

Sigma-Aldrich

H8891

Heparinase III from Flavobacterium heparinum

Sigma-Aldrich

Sigma-Aldrich

P7208

Pertussis toxin from Bordetella pertussis

assay

≥90% (SDS-PAGE)

assay

-

assay

-

assay

-

form

lyophilized powder

form

lyophilized solid

form

lyophilized powder

form

lyophilized powder

solubility

H2O: 10 mg/mL

solubility

-

solubility

-

solubility

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

200

composition

Protein, ~5% Lowry-TCA

composition

-

composition

-

composition

-

Application

Cholera Toxin from Vibrio cholerae has been used as a positive control in cAMP (cyclic AMP) assay for enterotoxins. It has been added as a supplement in cell culture media of primary tumors and epithelial cells.

Biochem/physiol Actions

Toxin consisting of an A subunit (27 kDa) surrounded by five B subunits (approximately 12 kDa each), which attach the toxin to ganglioside GM1 on the cell surface. The A subunit catalyzes ADP-ribosylation of the α-subunit of the stimulatory G protein (Gαs), reducing GTPase activity and activating the α-subunit. This activation of Gαs leads to an increase in the activity of adenylyl cyclase, resulting in increased levels of cAMP. Also ADP-ribosylates transducin in the eye rod outer segments, inactivating its GTPase activity. Cholera toxin has also been reported to ADP-ribosylate tubulin. Shown to be a potent mucosal vaccine adjuvant, inducing T helper cell type 2 responses by inhibiting the production of interleukin-12.

Features and Benefits

This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

Packaging

Package size based on protein content.

Physical form

Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide and sodium EDTA

Reconstitution

When reconstituted at 1 mg/mL in water, solution will contain 0.05 M Tris buffer salts, pH 7.5, 0.2 M NaCl, 0.003 M NaN3, and 0.001 M sodium EDTA. Store reconstituted solutions in the refrigerator.

Pictograms

Skull and crossbones
GHS06

Signal Word

Danger

Hazard Statements

H300 + H310 - H332 - H412

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 2 Oral - Acute Tox. 4 Inhalation - Aquatic Chronic 3

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

(enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service