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COLO 858

93052613

biological source

human skin

growth mode

Adherent

karyotype

Not specified

morphology

Fibroblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

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COLO 858 93052613

COLO 858

COLO 685 87061206

COLO 685

COLO 685 87061206

COLO 685

COLO 684 87061203

COLO 684

biological source

human skin

biological source

human uterus

biological source

human uterus

biological source

human uterus

growth mode

Adherent

growth mode

Suspension

growth mode

Suspension

growth mode

Suspension

karyotype

Adherent

karyotype

Suspension

karyotype

Suspension

karyotype

Suspension

morphology

Adherent

morphology

Suspension

morphology

Suspension

morphology

Suspension

products

Adherent

products

Suspension

products

Suspension

products

Suspension

receptors

Adherent

receptors

Suspension

receptors

Suspension

receptors

Suspension

Cell Line Origin

Human melanoma

Cell Line Description

Derived from a right axial lymph node of a 43 year old male with melanoma.COLO 839 (Sigma Catalogue number 93051119), COLO 858 (Sigma Catalogue number 93052613) and COLO 857 (Sigma Catalogue number 93051120) were all derived from the same 43 year-old male patient with malignant melanoma (see references below). This has been confirmed by STR profiling. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Application

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11,12
D16S539: 13
D5S818: 11
D7S820: 8,10
THO1: 10
TPOX: 8,11
vWA: 17,19

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 37°C; 5% CO2.

Other Notes

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Certificate of Analysis

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Certificate of Origin

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