CHP2NC

Imprint^® Ultra Chromatin Immunoprecipitation Kit, Without Controls

ChIP kit for maximum sensitivity, compatible with Next-Gen sequencing

Description

General description

The Imprint Ultra Chromatin IP Kit is Sigma′s second generation chromatin immunoprecipitation (ChIP) kit developed for maximum sensitivity and optimum next-generation sequencing results. It provides a complete solution for Chromatin Immunoprecipitation, including columns and reagents for DNA purification. The kit allows researchers to explore the genome-wide binding sites of low abundance transcription factors (TFs), as well as novel histone modifications. It is optimized for ChIP reactions with chromatin from 10⁶ cells (up to ~50 μg DNA), and can also be scaled up (or several preparations pooled) to accommodate 10⁸ cells for genome-wide binding studies in ChIP-chip and ChIP-Seq applications.

Application

• Suitable for downstream applications
• Individual target characterization to genome-wide profiling techniques
• Characterization of signal transduction pathways
• Verification of ChIp-chIP and ChIP-seq data

Features and Benefits

• Use successfully CHiP′ed DNA associated with low abundance, medium, and highly expressed transcription factors, as well as histone modifications
• Greater capacity - can be used over a wide range of cell numbers ranging from 2-10 × 10⁶
• Maximum sensitivity - capable of detecting low abundance transcription factors with as little as 2 × 10⁶ cells
• Employs DNA-Blocked "Staph-Seq" for IP (immunoprecipitation), minimizing contaminating Staph A DNA in downstream ChIP-Seq applications.

Legal Information

Imprint is a registered trademark of Sigma-Aldrich Co. LLC

Kit Components Only

• Sodium dodecyl sulfate solution, for molecular biology, 10% in 18 megohm water
• Monoclonal Anti-POLR2A antibody produced in mouse, clone 1F17, purified immunoglobulin, buffered aqueous solution

Kit Components Also Available Separately

• S6576 ChIP Next Gen Seq Sepharose^™
• I8896 IGEPAL^® CA-630, for molecular biology
• S5150 Sodium chloride solution, 5 M in H₂O, BioReagent, for molecular biology, suitable for cell culture
• W4502 Water, Nuclease-Free Water, for Molecular Biology
• I5006 IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder
• I5381 IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powder
• A7638 Bovine Serum Albumin, lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)
A8456 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, ≥97.0% (HPLC)
D9156 Deoxyribonucleic acid, single stranded from salmon testes, For hybridization
M7023 Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
P8340 Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution
R4642 Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

See All (12)

Safety Information

• Pictograms: GHS02,GHS03,GHS05,GHS07,GHS08,GHS09
• Signal Word: Danger
• Hazard Statements: H226 - H271 - H290 - H302 - H314 - H334 - H336 - H410
• Precautionary Statements: P210 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Ox. Liq. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
• Target Organs: Central nervous system
• Storage Class Code: 5.1A - Strongly oxidizing hazardous materials
• WGK: WGK 3