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3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride

for Immunohistology

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General description

3,3′-Diaminobenzidine (DAB) is a chromogenic substrate for peroxidase enzyme. It is converted to an insoluble precipitate after oxidation by peroxidase.


3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride has been used:
  • in the detection of hydrogen peroxide in rice leaves
  • as a substrate for rabbit peroxidase in immunohistochemistry of mice brain sections
  • in immunocytochemistry of muscle-derived stem cells (MDSCs)

The DAB (3,3′-Diaminobenzidine tetrahydrochloride) Two-component Enhanced Liquid Substrate System is a precipitating substrate system for peroxidase. DAB Two-component Liquid Substrate System produces a brown precipitate for use in immunohistological and immunoblotting staining procedures.


Stable for up to 18 mo. when stored at 2-8 °C in the original container.


Each DAB Two-component Liquid Substrate System will provide 100 mL of the DAB substrate solution.

Kit Components Only

Product No.

  • 3, 3′-Diaminobenzidine enhanced liquid substrate system, solution B, for immuno-histochemistry 100 mL


Health hazard

Signal Word


Hazard Statements

Hazard Classifications

Carc. 1B - Muta. 2

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects



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Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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