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D6883

Sigma-Aldrich

2′,7′-Dichlorodihydrofluorescein diacetate

≥97%

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Synonym(s):
2′,7′-Dichlorofluorescin diacetate, 2′,7′-Dichlorodihydrofluorescein diacetate
Empirical Formula (Hill Notation):
C24H16Cl2O7
CAS Number:
Molecular Weight:
487.29
Beilstein:
4610273
MDL number:
PubChem Substance ID:
NACRES:
NA.77

Quality Level

Assay

≥97%

form

powder

storage temp.

−20°C

SMILES string

CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C(c2cc1Cl)c4ccccc4C(O)=O

InChI

1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)

InChI key

PXEZTIWVRVSYOK-UHFFFAOYSA-N

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This Item
D6665410217287810
vibrant-m

D6665

2′,7′-Dichlorofluorescein

vibrant-m

410217

2′,7′-Dichlorofluorescein

assay

≥97%

assay

~90% (TLC)

assay

-

assay

≥94% (HPLC)

form

powder

form

powder

form

powder

form

crystalline solid

storage temp.

−20°C

storage temp.

room temp

storage temp.

room temp

storage temp.

−20°C

General description

2′,7′-Dichlorofluorescin diacetate is a cell-permeable non-fluorescent probe.It is de-esterified intracellularly and turns to highly fluorescent 2′,7′-dichlorofluorescein upon oxidation.

Application

2′,7′-Dichlorofluorescin diacetate is a cell-permeable non-fluorescent probe. 2′,7′-Dichlorofluorescin diacetate is de-esterified intracellularly and turns to highly fluorescent 2′,7′-dichlorofluorescein upon oxidation.
Applications include sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay.
2′,7′-Dichlorofluorescin diacetate is suitable for use in:
  • sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism
  • microplate assay for detecting oxidative products in phagocytic cells
  • quantitative multiwell myeloid differentiation assay

Storage and Stability

This product should be stored in powdered format at -20°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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