Merck
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D7295

Sigma-Aldrich

Deoxynucleotide Mix, 10 mM

Molecular Biology Reagent

Synonym(s):
dNTP mix, 10mM dNTP mix, dNTPs
NACRES:
NA.52

Quality Level

form

liquid

concentration

10 mM

color

colorless

application(s)

agriculture

foreign activity

DNase, RNase, none detected

shipped in

dry ice

storage temp.

−20°C

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General description

Nucleotides are organic molecules that serve as the monomers, or subunits, of nucleic acids (like DNA and RNA). The building blocks of nucleic acids, nucleotides consist of a nitrogenous base (purine or pyrimidine), a five-carbon sugar (ribose or deoxyribose), and at least one phosphate group. A nucleoside along with a phosphate group yields a nucleotide. The Deoxynucleotide mix is a convenient premixed dNTP solution containing 10 mM each of UltraPure dATP, dCTP, dGTP, and TTP sodium salts in high-quality molecular biology grade water. One µL is sufficient for a standard 50 µL PCR reaction.

Application

dNTP Mix has been used:

  • in the PCR amplification of genomic DNA isolated from insect, fungi, virus, human,
  • in reverse transcription of total RNA to cDNA.
  • as a component of the DNA amplification mixture for polymerase chain reaction (PCR)
  • routine and long PCR
  • manual and automated DNA sequencing
  • cDNA synthesis and labeling reactions

Packaging

0.2, 20×0.2, 0.5 mL in poly bottle

Features and Benefits

  • Purity of each dNTP: Minimum 99%
  • Conveniently formulated; 1 μL is used per 50 μL PCR
  • Equimolar amounts of each dNTP means less pipetting
  • Minimize risk of contamination in PCR
  • UltraPure dNTPs can help maximize consistency and yields in critical PCR reactions

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What are the components to Product D7295, Deoxynucleotide Mix?

    The D7295 contains 10mM of each dATP, dCTP, dGTP and TTP in molecular biology grade water.  This is for a total concentration of 40 mM.

  6. Does Product No. D7295, Deoxynucleotide Mix contain dTTP or TTP?

    The product contains Thymidine 5´-triphosphate sodium salt.  Thymidine has a deoxy sugar, so it is a deoxythymidine.  

  7. How much Product D7295, Deoxynucleotide Mix, is needed for a PCR reaction?

    One μl is sufficient for a standard 50 μl PCR reaction.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Protocols

Long and Accurate PCR Amplification of DNA (D8045)

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.

Low Contaminant Amplification of DNA Using MTP™ Taq DNA Polymerase

Method for bacterial genome analysis and detection of pathogens. Minimize false positive PCRs through lab design and reagents tested for use in bacterial PCR applications.

Amplification of Damaged DNA with Restorase DNA Polymerase (R1028)

Method for amplification of DNA from damaged DNA sources. Particularly useful for DNA extracted from old samples.

Amplification of DNA Using Jumpstart™ Taq DNA Polymerase (D4184)

Hot Start Taq Polymerase protocol to reduce non-specific amplification, with MgCl2 Optimization

See All

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