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DNB300

Sigma-Aldrich

GenElute Urine Cell-Free DNA Purification Mini Kit

greener alternative
NACRES:
NA.52

greener alternative product characteristics

Designing Safer Chemicals
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative category

Aligned

shipped in

ambient

storage temp.

room temp

General description

Sigma′s GenElute Urine Cell-Free DNA Purification Mini Kit provides a fast, reliable and simple procedures for isolating cell-free circulating DNA (cfc-DNA) from various amounts of urine ranging from 250 μL up to 2 mL. Purification is based on spin column chromatography that uses Sigma′s proprietary resin separation matrix. The kits are designed to isolate all sizes of cfc-DNA from either fresh or frozen urine samples. Moreover, these kits allow the user to elute the purified cfc-DNA into a flexible elution volume ranging from 25 μL to 50 μL. The purified urine cfc-DNA is eluted in an elution buffer that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, and NGS.

  • Isolate all sizes of circulating DNA from fresh, preserved or frozen urine samples
  • Isolate viral DNA
  • Versatile urine input volumes (250 μL - 2 mL)
  • Concentrate circulating DNA into a flexible elution volume ranging from (50 μL - 100 μL)
  • Isolate inhibitor-free cell-free circulating DNA
  • Purify high-quality DNA in 15-20 minutes

Application

  • PCR
  • qPCR
  • Southern blot
  • Methylation-sensitive PCR
  • CpG array
  • Restriction enzyme digestions
  • Viral detection
  • Bacterial detection
  • Microarray
  • NGS

Unit Definition

Preps

Other Notes

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2

Storage Class Code

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 3

Certificate of Analysis

Certificate of Origin

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