EPI018

Sigma-Aldrich

Sirtuin Activity Assay Kit (Fluorometric)

sufficient for 100 fluorometric tests

NACRES:
NA.84

usage

sufficient for 100 fluorometric tests

detection method

fluorometric

storage temp.

−20°C

General description

Sirtuins are a class of proteins that possess either histone deacetylase or mono-ribosyltransferase activity. Sirtuins are localized in the cytoplasm, nucleus, nucleolus as well as mitochondria. They are associated with aging, cellular protection, sugar metabolism and cell cycle regulation. Unlike other known protein deacetylases, which simply hydrolyze acetyl-lysine residues, the sirtuin-mediated deacetylation reaction hydrolyzes acetyl-lysine and NAD. This hydrolysis yields the deacetylated substrate, O-acetyl-ADP-ribose and nicotinamide, itself an inhibitor of sirtuin activity. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. 

Suitability

Suitable for the detection of Sirtuin Activity in purified recombinant protein, cell and tissue lysate, nuclear extract, mitochondria and immunoprecipitated samples

Principle

In the Sirtuin Activity Assay Kit, the acetylated p53-AFC substrate is deacetylated by Sirtuins in the presence of NAD+ to generate the deacetylated p53-AFC substrate, nicotinamide and O-Acetyl-ADP Ribose. Cleavage of the deacetylated p53-AFC substrate by the Developer releases the fluorescent group, which is detected fluorometrically ((λex = 400/λem = 505 nm). HDAC′s also deacetylate the acetylated p53-AFC substrate. Trichostatin A is added to the reaction to specifically inhibit HDAC′s in samples. This kit provides a rapid, simple, sensitive, and reliable test to measure Sirtuin Activity in a variety of samples. The limit of quantification of the assay
is 0.06 μU of recombinant human SIRT6.

pictograms

CorrosionHealth hazard

signalword

Danger

hazcat

Eye Dam. 1 - Resp. Sens. 1 - Skin Sens. 1

storage_class_code

10 - Combustible liquids

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificate of Analysis

Certificate of Origin

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