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F7378

Sigma-Aldrich

Fluorescein diacetate

used as cell viability stain

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Synonym(s):
3,6-Diacetoxyfluoran, Di-O-acetylfluorescein
Empirical Formula (Hill Notation):
C24H16O7
CAS Number:
Molecular Weight:
416.38
Beilstein:
62575
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

form

powder

mp

200-203 °C (lit.)

solubility

acetone: 25 mg/mL

ε (extinction coefficient)

≥86000 at 488-494 nm in 0.1 M NaOH

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccccc45)c1

InChI

1S/C24H16O7/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24/h3-12H,1-2H3

InChI key

CHADEQDQBURGHL-UHFFFAOYSA-N

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This Item
C5041F2456343209
Fluorescein diacetate used as cell viability stain

Sigma-Aldrich

F7378

Fluorescein diacetate

Fluorescein Diacetate Stains living cells green. Substrate for esterases. Suitable for use in cell viability assays. Removal of the acetate groups by cellular esterases results in fluorescence in cells with intact membranes.

Sigma-Aldrich

343209

Fluorescein Diacetate

mp

200-203 °C (lit.)

mp

-

mp

320 °C (lit.)

mp

-

solubility

acetone: 25 mg/mL

solubility

chloroform/ethanol (1:1): 9.80-10.20 mg/mL, clear, colorless to light yellow

solubility

-

solubility

ethanol: soluble

ε (extinction coefficient)

≥86000 at 488-494 nm in 0.1 M NaOH

ε (extinction coefficient)

-

ε (extinction coefficient)

≥12000 at 282-286 nm, ≥39000 at 236-240 nm, ≥70000 at 488-492 nm

ε (extinction coefficient)

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

storage temp.

room temp

storage temp.

−20°C

storage temp.

room temp

storage temp.

2-8°C

General description

Fluorescein diacetate (FDA) is a non-fluorescent molecule, which is hydrolysed to fluorescent fluorescein in live cells. Dead cells cannot accumulate or hydrolyse FDA.

Application

Fluorescein diacetate has been used for the differentiation of live cells from dead cells.

Substrates

Lipase substrate

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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