G8898

Glycine

suitable for electrophoresis, ≥99%

• Synonym(s): Aminoacetic acid, Aminoethanoic acid, Glycocoll
• Linear Formula: NH₂CH₂COOH
• CAS Number: 56-40-6
• Molecular Weight: 75.07
• Beilstein: 635782
• EC Number: 200-272-2
• MDL number: MFCD00008131
• eCl@ss: 32160406
• PubChem Substance ID: 24895355

Properties

• Quality Level: 200
• Assay: ≥99%
• form: powder
• technique(s): electrophoresis: suitable; western blot: suitable
• impurities: ≤0.01% Insoluble matter
• color: white to off-white
• pKa (25 °C): (1) 2.35, (2) 9.60; 2.35
• mp: 240 °C (dec.) (lit.)
• solubility: H₂O: 200 mg/mL, clear, colorless to faintly yellow
• anion traces: chloride (Cl^-): ≤70 ppm
• cation traces: heavy metals (as Pb): ≤20 ppm
• absorption: ≤0.15 at 280 at 1 M
• SMILES string: NCC(O)=O
• InChI: 1S/C2H5NO2/c3-1-2(4)5/h1,3H2,(H,4,5)
• InChI key: DHMQDGOQFOQNFH-UHFFFAOYSA-N
• Gene Information: rat ... Grin2a(24409)

Description

General description

Glycine is a molecule with pI of 6.7, which is similar to the pH of stacking gel. It has advantage of low mobility, hydrophobicity and it does not associate with proteins. Glycine is a component of Tris-glycine and Tris-glycine-SDS (sodium dodecyl sulfate) running buffers for polyacrylamide gel electrophoresis. Glycine is also a component of Towbin′s transfer buffer for Western blots.

Application

• Glycine has been used for the preparation of gelatin composites scaffold.
• It has been used in the elution buffer to extract protein using HiTrap protein G column.
• It has been used in the SDS-sample, running and transfer buffers prepared for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and western blotting.

Biochem/physiol Actions

Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors.

Analysis Note

Tested for use in electrode buffers for PAGE and in transfer buffers for Western blots.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves