Merck
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G8898

Sigma-Aldrich

Glycine

suitable for electrophoresis, ≥99%

Synonym(s):
Aminoacetic acid, Aminoethanoic acid, Glycocoll
Linear Formula:
NH2CH2COOH
CAS Number:
Molecular Weight:
75.07
Beilstein:
635782
EC Number:
MDL number:
eCl@ss:
32160406
PubChem Substance ID:

Quality Level

Assay

≥99%

form

powder

technique(s)

electrophoresis: suitable
western blot: suitable

impurities

≤0.01% Insoluble matter

color

white to off-white

pKa (25 °C)

(1) 2.35, (2) 9.60
2.35

mp

240 °C (dec.) (lit.)

solubility

H2O: 200 mg/mL, clear, colorless to faintly yellow

anion traces

chloride (Cl-): ≤70 ppm

cation traces

heavy metals (as Pb): ≤20 ppm

absorption

≤0.15 at 280 at 1 M

SMILES string

NCC(O)=O

InChI

1S/C2H5NO2/c3-1-2(4)5/h1,3H2,(H,4,5)

InChI key

DHMQDGOQFOQNFH-UHFFFAOYSA-N

Gene Information

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This Item
1295800500468.16013
Glycine suitable for electrophoresis, ≥99%

Sigma-Aldrich

G8898

Glycine

Glycine United States Pharmacopeia (USP) Reference Standard

USP

1295800

Glycine

Glycine BioUltra, for molecular biology, ≥99.0% (NT)

Sigma-Aldrich

50046

Glycine

Glycine for synthesis

Sigma-Aldrich

8.16013

Glycine

assay

≥99%

assay

-

assay

≥99.0% (NT)

assay

≥99.0% (perchloric acid titration)

form

powder

form

-

form

crystalline

form

solid

technique(s)

electrophoresis: suitable, western blot: suitable

technique(s)

-

technique(s)

-

technique(s)

inhibition assay: suitable

impurities

≤0.01% Insoluble matter

impurities

-

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

impurities

-

color

white to off-white

color

-

color

white

color

-

mp

240 °C (dec.) (lit.)

mp

240 °C (dec.) (lit.)

mp

240 °C (dec.) (lit.)

mp

240 °C (dec.) (lit.)

General description

Glycine is a molecule with pI of 6.7, which is similar to the pH of stacking gel. It has advantage of low mobility, hydrophobicity and it does not associate with proteins. Glycine is a component of Tris-glycine and Tris-glycine-SDS (sodium dodecyl sulfate) running buffers for polyacrylamide gel electrophoresis. Glycine is also a component of Towbin′s transfer buffer for Western blots.

Application

  • Glycine has been used for the preparation of gelatin composites scaffold.
  • It has been used in the elution buffer to extract protein using HiTrap protein G column.
  • It has been used in the SDS-sample, running and transfer buffers prepared for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and western blotting.

Biochem/physiol Actions

Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors.

Analysis Note

Tested for use in electrode buffers for PAGE and in transfer buffers for Western blots.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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