G9277

Sigma-Aldrich

Guanidine thiocyanate

for molecular biology, ≥99%

Synonym(s):
Guanidinium rhodanide, Guanidinium thiocyanate
Linear Formula:
NH2C(=NH)NH2 · HSCN
CAS Number:
Molecular Weight:
118.16
Beilstein/REAXYS Number:
3563461
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.31

Quality Level

biological source

synthetic (organic)

grade

for molecular biology

assay

≥99%

form

powder or crystals

application(s)

RNA extraction: suitable

color

colorless to white,

mp

120-122 °C (lit.)

solubility

water: 100 mg/mL, clear, colorless

cation traces

Ba: ≤5 ppm
Fe: ≤5 ppm
Na: ≤0.5%
heavy metals (as Pb): ≤5 ppm

Featured Industry

Agriculture

foreign activity

DNase, RNase, none detected

SMILES string

SC#N.NC(N)=N

InChI

1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H

InChI key

ZJYYHGLJYGJLLN-UHFFFAOYSA-N

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Related Categories

Application

Guanidine thiocyanate has been used:
  • for the lysis of colorectal adenocarcinoma cell line prior to RNA isolation
  • as a PCR inhibitor in triplex mitochondrial DNA (mtDNA) qPCR assay
  • for DNA extraction from cerebrospinal fluid (CSF)

Chaotropic agent and strong denaturant; solubilizes cells.

Packaging

100, 250, 500 g in poly bottle

Biochem/physiol Actions

Guanidine thiocyanate is a denaturing agent and is used routinely in RNA isolation. It is used as a storage buffer for whole blood samples. Guanidine thiocyanate inactivates nucleases and is ideal for storing and freezing fecal samples for DNA studies. It is used in combination with phenol−chloroform in RNA extraction.

pictograms

CorrosionExclamation mark

signalword

Danger

hazcat

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

Supp Hazards

EUH032

storage_class_code

8B - Non-combustible, corrosive hazardous materials

WGK Germany

WGK 2

Flash Point F

Not applicable

Flash Point C

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Development of a universal internal positive control
Kavlick MF
Biotechniques, 65(5), 275-280 (2018)
Method for preparing DNA from feces in guanidine thiocyanate solution affects 16S rRNA-based profiling of human microbiota diversity
Hosomi K, et al.
Scientific reports, 7(1), 4339-4339 (2017)
Preservation of nucleic acid integrity in guanidine thiocyanate lysates of whole blood
Kravchenko AV, et al.
Russian Journal of Bioorganic Chemistry, 32(6), 547-551 (2006)
Direct isolation of poly (A)+ RNA from 4 M guanidine thiocyanate-lysed cell extracts using locked nucleic acid-oligo (T) capture
Jacobsen N, et al.
Nucleic Acids Research, 32(7), e64-e64 (2004)
Comprehensive PCR-based assay for detection and species identification of human herpesviruses
Johnson G, et al.
Journal of Clinical Microbiology, 38(9), 3274-3279 (2000)

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