G9422

β-Glycerophosphate disodium salt hydrate

BioUltra, suitable for cell culture, suitable for plant cell culture, ≥99% (titration)

• Synonym(s): BGP, Glycerol 2-phosphate disodium salt hydrate
• Linear Formula: (HOCH₂)₂CHOP(O)(ONa)₂ · xH₂O
• CAS Number: 154804-51-0
• Molecular Weight: 216.04 (anhydrous basis)
• EC Number: 212-464-3
• MDL number: MFCD00149082
• PubChem Substance ID: 329799988
• NACRES: NA.25

Properties

• product line: BioUltra
• Quality Level: 400
• assay: ≥99% (titration)
• form: powder
• technique(s): cell culture | mammalian: suitable; cell culture | plant: suitable
• impurities: ≤0.1% Insoluble matter; ≤0.5 mol % L-α-isomer
• mp: 102-104 °C (lit.)
• anion traces: chloride (Cl^-): ≤0.05%; sulfate (SO₄^2-): ≤0.05%
• cation traces: Al: ≤0.001%; Ca: ≤0.001%; Cu: ≤0.0005%; Fe: ≤0.001%; K: ≤0.005%; Mg: ≤0.0005%; Pb: ≤0.001%; Zn: ≤0.0005%
• SMILES string: O.[Na+].[Na+].OCC(CO)OP([O-])([O-])=O
• InChI: 1S/C3H9O6P.2Na.H2O/c4-1-3(2-5)9-10(6,7)8;;;/h3-5H,1-2H2,(H2,6,7,8);;;1H2/q;2*+1;/p-2
• InChI key: ROPZSVKNEIIIDE-UHFFFAOYSA-L

Description

Application

β-Glycerophosphate is a classical serine-threonine phosphatase inhibitor used in kinase reaction buffers. BGP is often used in combination with other phosphatase/protease inhibitors for broad spectrum inhibition. It functions as an organic phosphate donor and has been used in culture media for mesenchymal stem cell differentiation to osteoblast-type cells. BGP is also used to buffer M17 media for Lactococcus culture in recombinant protein expression.

Initiates differentiation of bone-marrow-derived stem cells embedded in a natural collagen/chitosan gel designed for tissue engineering.

Packaging

10, 50, 100, 500 g in poly bottle

Quality

Low inorganic phosphate content. Mixtures of α- and β-glycerophosphate have historically been used in microbiological media but high levels of inorganic (free) phosphate has been associated with the irregular growth of bacteria and unwanted precipitates.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable