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Sephadex® G-50

Fine, Cytiva, pack of 100 g

CAS Number:
MDL number:

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.


autoclavable In wet form (pH 7.0) at 120°C for 30 min


pack of 100 g




flow rate


cross-linked dextran


1 g swells to 9-11 mL

particle size

20-80 μm (dry)

cleaning in place


working range


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General description

Sephadex® G-50 Fine is well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. The Fine´s small bead size give higher efficiency.

Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations at where high flow rates and low operating pressures are required.

Features and Benefits

  • Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  • Suitable for purification of DNA from small molecules by gel filtration
  • Small bead size for shorter diffusion distances
  • Classic gel filtration medium.

Storage and Stability

Store at 4 to 30 °C ()

Analysis Note

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Legal Information

Sephadex is a registered trademark of Cytiva

Storage Class Code

13 - Non Combustible Solids

Certificate of Analysis

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Certificate of Origin

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