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shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
feature
autoclavable In wet form (pH 7.0) at 120°C for 30 min
packaging
pack of 100 g
manufacturer/tradename
Cytiva
parameter
flow rate
matrix
cross-linked dextran
swelling
1 g swells to 9-11 mL
particle size
20-80 μm (dry)
(wet)
cleaning in place
2-13
working range
2-10
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General description
Sephadex® G-50 Fine is well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. The Fine´s small bead size give higher efficiency.
Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations at where high flow rates and low operating pressures are required.
Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations at where high flow rates and low operating pressures are required.
Features and Benefits
- Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
- Suitable for purification of DNA from small molecules by gel filtration
- Small bead size for shorter diffusion distances
- Classic gel filtration medium.
Storage and Stability
Store at 4 to 30 °C ()
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Sephadex is a registered trademark of Cytiva
Storage Class Code
13 - Non Combustible Solids
Certificate of Analysis
Enter Lot Number to search for Certificate of Analysis (COA).
Certificate of Origin
Enter Lot Number to search for Certificate of Origin (COO).
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