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H7166

Sigma-Aldrich

Interleukin-3 human

IL-3, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

Synonym(s):
IL-3
MDL number:
NACRES:
NA.77

biological source

human

Quality Level

recombinant

expressed in HEK 293 cells

assay

>95% (SDS-PAGE)

form

lyophilized powder

potency

≤2 ng/mL EC50

quality

endotoxin tested

mol wt

dimer 17-45 kDa (glycosylated)

packaging

pkg of 10 μg

manufacturer/tradename

HumanZyme

technique(s)

cell culture | mammalian: suitable

impurities

<1 EU/μg

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... IL3(3562)

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Packaging

10 μg in poly bottle

Biochem/physiol Actions

Interleukin-3 (IL-3) is a multifunctional protein, originally called colony forming unit-stimulating activity (CFU-SA), and is produced by activated T lymphocytes. IL-3 supports the formation of multilineage colonies in the early development of multipotent hematopoietic progenitor cells. IL-3 has been shown to induce colony formation of macrophages, neutrophils, mast cells, and megakaryocytes from agar-suspended bone marrow cells. IL-3 also interacts with IL-2 to stimulate growth of T lymphocytes and to induce IgG secretion from activated B cells. Other synonyms for IL-3 include pan-specific hemopoietin, multicolony stimulating factor, mast cell growth factor, burst promoting activity, histamine-producing cell stimulating factor, P cell stimulating factor, and WEHI-3 factor.

Physical form

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Preparation Note

HumanKine Interleukin-3 (IL-3), expressed in human HEK 293 cells, is a glycosylated monomer with an apparent molecular mass of 17-45 kDa due to glycosylation, which is absent when this cytokine is expressed in E. coli. Production in human HEK 293 cells offers authentic glycosylation which contributes to stability in cell growth media and other applications.

Analysis Note

The activity was determined by the dosedependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

SDS

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