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H7166

Sigma-Aldrich

Interleukin-3 human

IL-3, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

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Synonym(s):
IL-3
MDL number:
NACRES:
NA.77

biological source

human

Quality Level

recombinant

expressed in HEK 293 cells

Assay

>95% (SDS-PAGE)

form

lyophilized powder

potency

≤2 ng/mL EC50

quality

endotoxin tested

mol wt

dimer 17-45 kDa (glycosylated)

packaging

pkg of 10 μg

manufacturer/tradename

HumanZyme

technique(s)

cell culture | mammalian: suitable

impurities

<1 EU/μg

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... IL3(3562)

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This Item
I1646H7666SCU0001
Sigma-Aldrich

Sigma-Aldrich

H7166

Interleukin-3 human

Sigma-Aldrich

Sigma-Aldrich

I1646

Interleukin-3 human

Sigma-Aldrich

Sigma-Aldrich

H7666

Interleukin-12 human

Interleukin-6 human recombinant, expressed in HEK 293 cells, suitable for cell culture

Sigma-Aldrich

SCU0001

Interleukin-6 human

recombinant

expressed in HEK 293 cells

recombinant

expressed in E. coli

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

assay

>95% (SDS-PAGE)

assay

≥97% (HPLC and SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

form

lyophilized powder

form

lyophilized powder

form

lyophilized

form

lyophilized powder

potency

≤2 ng/mL EC50

potency

≤1.0 ng/mL ED50/EC50

potency

≤2 ng/mL EC50

potency

0.2-2 ng/mL EC50

mol wt

dimer 17-45 kDa (glycosylated)

mol wt

15 kDa

mol wt

dimer 57 kDa (glycosylated)

mol wt

calculated mol wt 20.8 kDa (183 amino acids glycosylated protein)

Biochem/physiol Actions

Interleukin-3 (IL-3) is a multifunctional protein, originally called colony forming unit-stimulating activity (CFU-SA), and is produced by activated T lymphocytes. IL-3 supports the formation of multilineage colonies in the early development of multipotent hematopoietic progenitor cells. IL-3 has been shown to induce colony formation of macrophages, neutrophils, mast cells, and megakaryocytes from agar-suspended bone marrow cells. IL-3 also interacts with IL-2 to stimulate growth of T lymphocytes and to induce IgG secretion from activated B cells. Other synonyms for IL-3 include pan-specific hemopoietin, multicolony stimulating factor, mast cell growth factor, burst promoting activity, histamine-producing cell stimulating factor, P cell stimulating factor, and WEHI-3 factor.

Physical form

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Preparation Note

HumanKine Interleukin-3 (IL-3), expressed in human HEK 293 cells, is a glycosylated monomer with an apparent molecular mass of 17-45 kDa due to glycosylation, which is absent when this cytokine is expressed in E. coli. Production in human HEK 293 cells offers authentic glycosylation which contributes to stability in cell growth media and other applications.

Analysis Note

The activity was determined by the dosedependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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