I0516

Insulin solution from bovine pancreas

BioReagent, 10 mg/mL insulin in 25  mM HEPES, pH 8.2, sterile-filtered, suitable for cell culture

• Synonym(s): Bovine insulin, Cell culture grade insulin
• CAS Number: 11070-73-8
• MDL number: MFCD00131380
• NACRES: NA.77

Properties

• sterility: sterile-filtered
• Quality Level: 400
• product line: BioReagent
• form: solution
• concentration: 10 mg/mL insulin in 25 mM HEPES, pH 8.2
• technique(s): cell culture | mammalian: suitable; single cell analysis: suitable
• impurities: endotoxin, tested
• pH: 7.9-8.5
• UniProt accession no.: P01317
• shipped in: ambient
• storage temp.: 2-8°C
• InChI: 1S/2C97H151N25O34S4/c2*1-12-46(10)77(120-72(131)35-98)96(154)122-76(45(8)9)94(152)107-55(24-28-74(134)135)79(137)104-53(22-26-69(100)128)82(140)116-66(40-159)93(151)118-64(38-157)90(148)103-47(11)78(136)114-63(37-124)89(147)121-75(44(6)7)95(153)119-67(41-160)92(150)115-62(36-123)88(146)109-57(30-43(4)5)84(142)110-58(31-48-13-17-50(125)18-14-48)85(143)105-52(21-25-68(99)127)80(138)108-56(29-42(2)3)83(141)106-54(23-27-73(132)133)81(139)112-60(33-70(101)129)87(145)111-59(32-49-15-19-51(126)20-16-49)86(144)117-65(39-158)91(149)113-61(97(155)156)34-71(102)130/h2*13-20,42-47,52-67,75-77,123-126,157-160H,12,21-41,98H2,1-11H3,(H2,99,127)(H2,100,128)(H2,101,129)(H2,102,130)(H,103,148)(H,104,137)(H,105,143)(H,106,141)(H,107,152)(H,108,138)(H,109,146)(H,110,142)(H,111,145)(H,112,139)(H,113,149)(H,114,136)(H,115,150)(H,116,140)(H,117,144)(H,118,151)(H,119,153)(H,120,131)(H,121,147)(H,122,154)(H,132,133)(H,134,135)(H,155,156)/t2*46-,47-,52-,53-,54-,55-,56-,57-,58-,59-,60-,61-,62-,63-,64-,65-,66-,67-,75-,76-,77-/m00/s1
• InChI key: XYPANFGXFLOTDK-CJLNJPJHSA-N
• Gene Information: bovine ... INS(280829) ; cow ... INS(280829)

Description

Application

Insulin solution from bovine pancreas has been used:

• to study the effect of exogenous insulin on plasma concentrations of glucose, insulin, insulin-like growth factor I (IGF-I), and progesterone (P4) in pubertal beef heifers
• to study the insulin responsiveness of the placenta during pregnancy complications using primary human trophoblast cells and placental villous explants
• to study the effect of insulin receptor signaling dendritic spine density in neonatal mouse hippocampal neurons in vitro

Biochem/physiol Actions

Insulin is a major glucose-lowering hormone, insufficiency of which causes hyperglycemia observed in type 2 diabetes.

Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat.

Physical form

stable 2-8 °C

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves