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I2002

Isocitric Dehydrogenase (NADP) from porcine heart

Type IV, buffered aqueous glycerol solution, 3-20 units/mg protein

Synonym(s):

threo-Ds-Isocitrate: NADP+ oxidoreductase, ICDH, IDH

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25 MG

CHF 147.00

100 MG

CHF 486.00

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CHF 1’050.00

500 MG

CHF 1’770.00

CHF 147.00

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About This Item

CAS Number:
9028-48-2
EC Number:
1.1.1.42 (BRENDA, IUBMB)
EC Number:
200-289-5
MDL number:
MFCD00131420
UNSPSC Code:
12352204
NACRES:
NA.54

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type

Type IV

form

buffered aqueous glycerol solution

quality

≤ 0.2% of main activity is obtained with NAD as cofactor.

specific activity

3-20 units/mg protein

composition

Protein biuret

shipped in

wet ice

storage temp.

2-8°C

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1 of 4

This Item
I1877M2634SAB2501739
Isocitric Dehydrogenase (NADP) from porcine heart Type IV, buffered aqueous glycerol solution, 3-20 units/mg protein

Sigma-Aldrich

I2002

Isocitric Dehydrogenase (NADP) from porcine heart

Isocitric Dehydrogenase (NADP) from porcine heart Type I, 0.5-3.0 unit/mg solid (plus numerous enzyme activities associated with porcine heart)

Sigma-Aldrich

I1877

Isocitric Dehydrogenase (NADP) from porcine heart

Malic Dehydrogenase from porcine heart buffered aqueous glycerol solution, 600-1000 units/mg protein (biuret)

Sigma-Aldrich

M2634

Malic Dehydrogenase from porcine heart

Anti-IDH3G antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

SAB2501739

Anti-IDH3G antibody produced in goat

specific activity

3-20 units/mg protein

specific activity

0.5-3.0 unit/mg solid (plus numerous enzyme activities associated with porcine heart)

specific activity

600-1000 units/mg protein (biuret)

specific activity

-

form

buffered aqueous glycerol solution

form

solid

form

buffered aqueous glycerol solution

form

buffered aqueous solution

shipped in

wet ice

shipped in

-

shipped in

wet ice

shipped in

wet ice

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

type

Type IV

type

Type I

type

-

type

-

Application

Isocitric dehydrogenase (IDH) has been used:
  • for in vitro activity assay to study the effects of thorium on citric acid cycle enzymes in the mitochondrial respiration pathway[1]
  • as a standard to measure the impact of temperature on nicotinamide diphosphate (NADP)-IDH activity[2]
  • in isocitrate assay[3]

Biochem/physiol Actions

Isocitrate dehydrogenase (IDH) is a homodimeric enzyme and participates in several processes such as adapting to hypoxia, histone demethylation, and DNA modification. This enzyme has three isoforms like IDH1, IDH2, and IDH3.[4]

Physical form

Solution in 50% glycerol in EDTA buffer salts, pH 6.0

Other Notes

One unit will convert 1.0 μmole of isocitrate to α-ketoglutarate per min at pH 7.4 at 37 °C.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000502290
0000502291
0000502293
0000502293
0000502290

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Icksoo Lee et al.
Biochimica et biophysica acta, 1802(2), 275-283 (2009-10-20)
Noonan syndrome (NS) is an autosomal dominant disorder, and a main feature is congenital heart malformation. About 50% of cases are caused by gain-of-function mutations in the tyrosine phosphatase SHP2/PTPN11, a downstream regulator of ERK/MAPK. Recently it was reported that
Avtar Singh et al.
The Journal of biological chemistry, 278(35), 33208-33216 (2003-06-07)
Transhydrogenase couples the reduction of NADP+ by NADH to inward proton translocation across mitochondrial and bacterial membranes. The coupling reactions occur within the protein by long distance conformational changes. In intact transhydrogenase and in complexes formed from the isolated, nucleotide-binding
Sergiy M Nadtochiy et al.
The Journal of biological chemistry, 291(38), 20188-20197 (2016-08-12)
2-Hydroxyglutarate (2-HG) is an important epigenetic regulator, with potential roles in cancer and stem cell biology. The d-(R)-enantiomer (d-2-HG) is an oncometabolite generated from α-ketoglutarate (α-KG) by mutant isocitrate dehydrogenase, whereas l-(S)-2-HG is generated by lactate dehydrogenase and malate dehydrogenase
Erdem M Terzi et al.
Science advances, 7(22) (2021-05-28)
Intracellular iron levels are strictly regulated to support homeostasis and avoid iron-mediated ROS production. Loss of iron-sulfur cluster (ISC) synthesis can increase iron loading and promote cell death by ferroptosis. Iron-responsive element-binding proteins IRP1 and IRP2 posttranscriptionally regulate iron homeostasis.
M Ryan Smith et al.
Redox biology, 8, 136-148 (2016-01-18)
Many cancer cells follow an aberrant metabolic program to maintain energy for rapid cell proliferation. Metabolic reprogramming often involves the upregulation of glutaminolysis to generate reducing equivalents for the electron transport chain and amino acids for protein synthesis. Critical enzymes
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