Acute Tox. 2 Oral
6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
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One can try to make a 20 mg/mL solution at 70-80°C in saline, with vortexing and warming. However, the solution will be hazy, because of the nature of LPS to form micelles in solutions like water or PBS.
Different extraction methods for LPS leave different levels of potential nucleic acid and protein contaminations. Phenol extraction can leave up to 60% RNA and <1% protein present.
We have not determined the molecular weight of this item. One reference, J. Biol. Chem., 250, 2911 (1975), states approximate values of 90,000 and 0.9-1.5 million, by both sedimentation velocity and gel filtration, for two forms of LPS that may be present.
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