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Lipopolysaccharides from Escherichia coli O111:B4

Ready Made solution, 1 mg/mL

MDL number:

biological source

Escherichia coli (O111:B4)

Quality Level


aqueous solution


1 mg/mL

shipped in

wet ice

storage temp.


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Lipopolysaccharides from Escherichia coli O111:B4 has been used:
  • to stimulate an inflammatory response in porcine liver tissues in-vivo[1] and piglet ileum tissues ex vivo[2]
  • to stimulate Ex vivo natural killer(NK) cells[3] and hepatic B cells[4]
  • to induce arthritis in a standard rabbit model[5]

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

Lipopolysaccharides (LPS) are distributed on the outer layer of the membrane and in non-capsulated strains, present on the cell surface. LPS maintains the outer membrane integrity. In addition, it protects the cell from bile salts stress and lipophilic antibiotics.[6]

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificate of Origin

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