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M5655

Sigma-Aldrich

Thiazolyl Blue Tetrazolium Bromide

powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)

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Synonym(s):
3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, MTT, Methylthiazolyldiphenyl-tetrazolium bromide
Empirical Formula (Hill Notation):
C18H16BrN5S
CAS Number:
298-93-1
Molecular Weight:
414.32
Beilstein:
4081397
EC Number:
206-069-5
MDL number:
MFCD00011964
PubChem Substance ID:
24897030
NACRES:
NA.75

product line

BioReagent

Quality Level

200

Assay

≥97.5% (HPLC)

form

powder

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable

mp

195 °C (dec.) (lit.)

solubility

H2O: 5 mg/mL

storage temp.

2-8°C

SMILES string

[Br-].Cc1nc(sc1C)-[n+]2nc(nn2-c3ccccc3)-c4ccccc4

InChI

1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1

InChI key

AZKSAVLVSZKNRD-UHFFFAOYSA-M

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This Item
M21284759891.11714
Thiazolyl Blue Tetrazolium Bromide powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)

Sigma-Aldrich

M5655

Thiazolyl Blue Tetrazolium Bromide

Thiazolyl Blue Tetrazolium Bromide 98%

Sigma-Aldrich

M2128

Thiazolyl Blue Tetrazolium Bromide

MTT Membrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan.

Sigma-Aldrich

475989

MTT

3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide for biochemistry

Millipore

1.11714

3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide

assay

≥97.5% (HPLC)

assay

98%

assay

≥98% (silver nitrate titration)

assay

-

form

powder

form

powder

form

solid

form

solid

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

-

mp

195 °C (dec.) (lit.)

mp

195 °C (dec.) (lit.)

mp

-

mp

195 °C

solubility

H2O: 5 mg/mL

solubility

2-methoxyethanol: soluble 20 mg/mL, H2O: 5 mg/mL

solubility

PBS: 2 mg/mL

solubility

10 g/L

Application

Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.
Thiazolyl Blue Tetrazolium Bromide has been used as a colorimetric metabolic activity indicator in cell viability assays (MTT assay). It has also been used to determine cell proliferation.

Pictograms

Exclamation markHealth hazard
GHS07,GHS08

Signal Word

Warning

Hazard Statements

H315 - H319 - H335 - H341

Hazard Classifications

Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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