This product has not been validated for deparaffinization of formalin-fixed, paraffin-embedded samples. However, an article in "Applied Immunohistochemistry & Molecular Morphology" (2014 Sep;22(8):623-6) indicated that mineral oil appears to work well for deparaffinizing these samples and for DNA extraction.
M8410
Mineral oil
light oil, (neat)
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5 ML
CHF 37.00
100 ML
CHF 42.60
500 ML
CHF 75.10
1 L
CHF 138.00
About This Item
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Quality Level
sterility
non-sterile
form
light oil
storage condition
protect from light
refractive index
n20/D 1.467 (lit.)
density
0.84 g/mL at 25 °C (lit.)
format
neat
InChI key
AEOVEGJBKQQFOP-DDVLFWKVSA-L
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This Item | M3516 | 330779 | 330760 |
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form light oil | form light oil | form viscous liquid | form liquid (colorless) |
| density 0.84 g/mL at 25 °C (lit.) | density 0.84 g/mL at 25 °C (lit.) | density 0.833 g/mL at 25 °C | density 0.862 g/mL at 25 °C |
| refractive index n20/D 1.467 (lit.) | refractive index n20/D 1.467 (lit.) | refractive index n20/D 1.467 (lit.) | refractive index n20/D 1.467 (lit.) |
| sterility non-sterile | sterility - | sterility - | sterility - |
| storage condition protect from light | storage condition - | storage condition - | storage condition - |
Analysis Note
This material is tested as an overlay in microdrop embryo cultures. (All embryo tested materials are tested using mouse embryos.) Typically BCF1 x BDF1 hybrids are used. The oil is primarily used to prevent evaporation of the medium, particiularly when performing embryo manipulations.1 The procedure is as follows: 1-cell (BCF1 X BDF1) embryos were cultured in triplicate microdrops of embryo-tested HTF (Human Tubal Fluid) medium supplemented with 0.4% BSA. For a valid assay, at least 70% of 1-cell stage control embryos must develop to the blastocyst stage within 96 hours. For product release, at least 80% of 1-cell stage test embryos must develop to the blastocyst stage within 96 hours with the tested material.
Disclaimer
This product is not sterile. Mineral oil is difficult to sterilize. It can only be sterilized by careful filtration of the warmed oil. It is assumed antibiotics will be added to the cell culture medium. Otherwise, Prod. No. M5310 should be used, as it is sterile filtered.
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Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
No data available
Flash Point(C)
No data available
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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TIE: A Method to Electroporate Long DNA Templates into Preimplantation Embryos for CRISPR-Cas9 Gene Editing
Bagheri H, et al.
The CRISPR journal, 1(3), 223-229 (2018)
Minoru Kubo et al.
Nucleic acids research, 47(9), 4539-4553 (2019-03-16)
Next-generation sequencing technologies have made it possible to carry out transcriptome analysis at the single-cell level. Single-cell RNA-sequencing (scRNA-seq) data provide insights into cellular dynamics, including intercellular heterogeneity as well as inter- and intra-cellular fluctuations in gene expression that cannot
Insulin and branched-chain amino acid depletion during mouse preimplantation embryo culture programmes body weight gain and raised blood pressure during early postnatal life
Velazquez M
Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1864(2), 590-600 (2018)
Production of a gonadotropin-releasing hormone 2 receptor knockdown (GNRHR2 KD) swine line
Desaulniers AT, et al.
Transgenic research, 26(4), 567-575 (2017)
Tiffany Leidy-Davis et al.
Scientific reports, 8(1), 15028-15028 (2018-10-12)
Here, we describe an expansion of the typical DNA size limitations associated with CRISPR knock-in technology, more specifically, the physical extent to which mouse genomic DNA can be replaced with donor (in this case, human) DNA at an orthologous locus
Articles
Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture
Related Content
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Can M8410 Sigma-Aldrich Mineral oil be used for the deparaffinization of FFPE samples, and is it compatible with downstream histological or molecular analyses like immunohistochemistry or RNA/DNA extraction?
1 answer-
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Hello,I´m looking for a mineral oil for sulfur pattern production. It´s difficult to find an oil in which the content of sulfur compounds appears in the COA. Can you inform if there´s a possibility of sulfur contamination in the production of mineral oil?
1 answer-
The manufacturing process for this product is considered proprietary. Currently, there is no product option available that is tested for the presence of sulfur.
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ıs this suitable for preparation of carbon paste electrode?
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This material has not been validated in the process of making carbon paste electrodes. However, if the specifications (found below) meet your requirements, then there is no reason that they can't be used in this process.
https://www.sigmaaldrich.com/specification-sheets/990/164/M8410-BULK.pdfHelpful?
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Hello, Does this product have an odour? I want to use it for an olfactory memory assay, so needs to be odourless
1 answer-
According to Section 9 of the Safety Data Sheet, this is an odorless chemical.
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What is the composition of the oil? Where does it comes from?
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M8410 is a light oil. Mineral oil is a complex mixture of different hydrocarbons and its typical composition is as follows:
Paraffinic oils, based on n-alkanes, 15-60%
Naphthenic oils, based on cycloalkanes, 30%-60%
Aromatic oils, based on aromatic hydrocarbons, 3-30%
Light and heavy mineral oil differ in terms of composition, viscosity, and density.
Mineral oil with an alkyl chain carbon number of 15 to 25 is categorized as light oil whereas 25 to 50 as heavy oil.
Light oil has a density of 0.83–0.86 kg L-1 whereas heavy oil has 0.875–0.905 kg L-1
Light mineral oil is less occlusive than heavy mineral oil as it contains more cyclic (saturated) moleculesAdditional Links: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/326/294/m8410pis.pdf and a second document: https://www.sigmaaldrich.com/specification-sheets/990/164/M8410-BULK.pdf
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