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M8410

Sigma-Aldrich

Mineral oil

light oil, (neat)

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CAS Number:
EC Number:
MDL number:

Quality Level

sterility

non-sterile

form

light oil

storage condition

protect from light

refractive index

n20/D 1.467 (lit.)

density

0.84 g/mL at 25 °C (lit.)

format

neat

InChI key

AEOVEGJBKQQFOP-DDVLFWKVSA-L

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This Item
M3516M5310M5904
vibrant-m

M8410

Mineral oil

vibrant-m

M3516

Mineral oil

vibrant-m

M5310

Mineral oil

vibrant-m

M5904

Mineral oil

form

light oil

form

light oil

form

light oil

form

light oil

density

0.84 g/mL at 25 °C (lit.)

density

0.84 g/mL at 25 °C (lit.)

density

0.82-0.88 g/mL at 25 °C

density

0.84 g/mL at 25 °C (lit.)

refractive index

n20/D 1.467 (lit.)

refractive index

n20/D 1.467 (lit.)

refractive index

n20/D 1.467 (lit.)

refractive index

n20/D 1.467 (lit.)

sterility

non-sterile

sterility

-

sterility

sterile-filtered

sterility

-

storage condition

protect from light

storage condition

-

storage condition

protect from light

storage condition

-

Application

Mineral oil has been used as an overlay in microdrop embryo cultures.

Caution

This product is not sterile. Mineral oil is difficult to sterilize. It can only be sterilized by careful filtration of the warmed oil. It is assumed antibiotics will be added to the cell culture medium. Otherwise, Prod. No. M5310 should be used, as it is sterile filtered.

Analysis Note

This material is tested as an overlay in microdrop embryo cultures. (All embryo tested materials are tested using mouse embryos.) Typically BCF1 x BDF1 hybrids are used. The oil is primarily used to prevent evaporation of the medium, particiularly when performing embryo manipulations.1 The procedure is as follows: 1-cell (BCF1 X BDF1) embryos were cultured in triplicate microdrops of embryo-tested HTF (Human Tubal Fluid) medium supplemented with 0.4% BSA. For a valid assay, at least 70% of 1-cell stage control embryos must develop to the blastocyst stage within 96 hours. For product release, at least 80% of 1-cell stage test embryos must develop to the blastocyst stage within 96 hours with the tested material.

comparable product

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

No data available

Flash Point(C)

No data available

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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