MAK037

Sigma-Aldrich

NAD/NADH Quantitation Kit

sufficient for 100 colorimetric tests

Synonym(s):
NAD/NADH Assay Kit
eCl@ss:
32161000

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

storage temp.

−20°C

General description

Nicotinamide adenine dinucleotide (NAD) is an enzymatic cofactor involved in many redox reactions. NAD functions as an electron carrier, cycling between the oxidized (NAD) and reduced (NADH) forms. In addition to its role in redox reactions, NAD plays critical roles in ADP (adenosine diphosphate)-ribosylation reactions and as a substrate for sirtuins.

Application

NAD/NADH Quantitation Kit has been used to quantify NAD+/NADH influenced by SIRT1 (sirtuin 1) activity (associated with ischemia-reperfusion injury) in the presence of trimetazidine.

Suitability

Suitable for the detection of NADH and NAD in animal tissues (liver, kidney etc.), cell culture (adherent or suspension cells), serum or urine.

Principle

The NAD/NADH Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides, NAD (nicotinamide adenine dinucleotide) and NADH, and their ratio without the requirement to purify NAD/NADH from samples. This assay is specific for NAD and NADH, and does not detect NADP or NADPH. NADt (NAD and NADH) or NADH are quantified in a colorimetric assay (450 nm).

pictograms

Exclamation mark

signalword

Warning

hazcat

Eye Irrit. 2 - Skin Irrit. 2

storage_class_code

10 - Combustible liquids

Certificate of Analysis

Certificate of Origin

Frédérique Moyrand et al.
Eukaryotic cell, 7(12), 2069-2077 (2008-09-30)
The genome of the basidiomycete pathogenic yeast Cryptococcus neoformans carries two UDP-glucose epimerase genes (UGE1 and UGE2). UGE2 maps within a galactose cluster composed of a galactokinase homologue gene and a galactose-1-phosphate uridylyltransferase. This clustered organization of the GAL genes...
Mammalian sirtuins?emerging roles in physiology, aging, and calorie restriction.
Haigis M C and Leonard P G
Genes & Development, 20(21), 2913-2921 (2006)
Andrea M Lencina et al.
mBio, 9(4) (2018-07-05)
The opportunistic pathogen Streptococcus agalactiae is the major cause of meningitis and sepsis in a newborn's first week, as well as a considerable cause of pneumonia, urinary tract infections, and sepsis in immunocompromised adults. This pathogen respires aerobically if heme...
Deepanwita Banerjee et al.
BMC systems biology, 11(1), 51-51 (2017-04-28)
The leading edge of the global problem of antibiotic resistance necessitates novel therapeutic strategies. This study develops a novel systems biology driven approach for killing antibiotic resistant pathogens using benign metabolites. Controlled laboratory evolutions established chloramphenicol and streptomycin resistant pathogens...
Haiping Wang et al.
Nature immunology, 21(3), 298-308 (2020-02-19)
Depleting regulatory T cells (Treg cells) to counteract immunosuppressive features of the tumor microenvironment (TME) is an attractive strategy for cancer treatment; however, autoimmunity due to systemic impairment of their suppressive function limits its therapeutic potential. Elucidating approaches that specifically...
Articles
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We presents an article about the Warburg effect, and how it is the enhanced conversion of glucose to lactate observed in tumor cells, even in the presence of normal levels of oxygen. Otto Heinrich Warburg demonstrated in 1924 that cancer cells show an increased dependence on glycolysis to meet their energy needs, regardless of whether they were well-oxygenated or not.
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