Merck
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MAK170

Sigma-Aldrich

Glutamate Oxidase Assay Kit

sufficient for 200 fluorometric tests

EC Number:
NACRES:
NA.84

usage

sufficient for 200 fluorometric tests

detection method

fluorometric

storage temp.

−20°C

Related Categories

General description

Glutamate oxidase is an oxidoreductase that catalyzes the oxidative deamination of L-glutamate to ketoglutarate, ammonia and hydrogen peroxide.
Glutamate oxidase is an oxidoreductase that catalyzes the oxidative deamination of L-glutamate to ketoglutarate, ammonia, and hydrogen peroxide.

The Glutamate Oxidase Assay Kit provides a simple and direct procedure for measuring glutamate oxidase activity in a variety of samples such as solutions and cell extracts. Glutamate Oxidase activity is determined by a coupled enzyme assay, in which the oxidation of glutamic acid results in a fluorescent product (λex =540/λem = 590 nm) proportional to the glutamate oxidase activity present. One unit of glutamate oxidase activity is defined as the amount of enzyme that catalyzes the conversion of L-glutamic acid to 1.0 μmole a-ketoglutaric acid per minute at pH 7.4 at room temperature.

Suitability

Suitable for measuring glutamate oxidase activity in a variety of samples such as solutions and cell extracts.

Principle

The glutamate oxidase assay kit provides a simple and direct procedure for measuring glutamate oxidase activity in a variety of samples such as solutions and cell extracts. Glutamate oxidase activity is determined by a coupled enzyme assay, in which the oxidation of glutamic acid results in a fluorescent product (λex =540/λem = 590 nm) proportional to the glutamate oxidase activity present. One unit of glutamate oxidase activity is defined as the amount of enzyme that catalyzes the conversion of L-glutamic acid to 1.0μmole α-ketoglutaric acid per minute at pH 7.4 at room temperature.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

SDS

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