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MAK176

Sigma-Aldrich

Granzyme B Activity Assay Kit

Sufficient for 100 Fluorometric tests

Synonym(s):
CTLA1, GZMB Activity Assay Kit
EC Number:
NACRES:
NA.84

usage

sufficient for 100 fluorometric tests

detection method

fluorometric

storage temp.

−20°C

Gene Information

human ... GZMB(3002)
mouse ... GZMB(14939)
rat ... GZMB(171528)

Related Categories

General description

GZMB codes for granzyme B.
Granzyme B (gzm B) is a peptidase that induces cytotoxic T lymphocyte (CTL)-mediated DNA fragmentation and apoptosis in allogeneic target cells. It cleaves after aspartic acid residues in substrates. Granzyme B-mediated release of mitochondrial cell death factors are required for the activation of caspase-3. Furthermore, the degradation of granzyme B is known to decrease the susceptibility of cancer cells to NK-cell-mediated lysis during hypoxia. Granzyme B activity assays can be used for studying T cell immunity, CTL responses, cell-mediated cytotoxicity, and apoptosis.
Gzm B is a pro-apoptotic member of the granzyme family. It is a neutral serine protease with Aspase activity. Gzm B is expressed exclusively in the granules of activated cytolytic T cells, natural killer cells and lymphokine-activated killer cells.

Suitability

Suitable for the detection of granzyme B activity in biological samples and for the characterization of granzyme B inhibitors.

Principle

Granzyme B activity is determined using an enzyme-catalyzed reaction in which the non-fluorescent substrate, Ac-IEPD-AFC, is hydrolyzed resulting in the release of AFC (λex = 380/λem = 500 nm) proportional to the enzymatic activity present. One unit of granzyme B is the amount of enzyme that hydrolyzes 1 pmole of the substrate per minute at 37 °C.

Kit Components Only

Product No.
Description

  • Granzyme B Assay Buffer

  • Granzyme B Substrate

  • Granzyme B Positive Control

  • AFC Standard, 1 mM

Storage Class Code

10 - Combustible liquids

Certificate of Analysis

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Certificate of Origin

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