Merck
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MAK204

Sigma-Aldrich

Enteropeptidase Activity Assay Kit

Sufficient for 100 Fluorometric tests

Synonym(s):
Enterokinase Activity Assay Kit
EC Number:
NACRES:
NA.84

detection method

fluorometric

storage temp.

−20°C

Related Categories

General description

Enteropeptidase (Enterokinase; EC 3.4.21.9) is an enzyme that converts trypsinogen to trypsin, the activity of which is required for the activation of chymotrypsin and procarboxypeptidases. It has an important role in food digestion and may serve as a marker of differentiated enterocytes and goblet cells.

Suitability

Suitable for the measurement of enteropeptidase/enterokinase activity in biological samples or purified preparations.

Principle

The Enteropeptidase/Enterokinase Activity Assay Kit is a rapid method to measure enteropeptidase activity in biological samples. Enteropeptidase activity is measured by cleaving a synthetic 7-amino-4-trifluoromethylcoumarin (AFC)-tagged peptide substrate containing the recognition sequence for enteropeptidase. This yields AFC, a fluorescent product (λex = 380/ λem = 500 nm), proportional to the enzymatic activity present. One unit of enteropeptidase is the amount of enzyme that generates 1.0 nmole of AFC per minute at room temperature.

Kit Components Only

Product No.
Description

  • Enteropeptidase Assay Buffer

  • Enteropeptidase Substrate, 10 mM in DMSO

  • Human Enteropeptidase

  • AFC Standard, 1 mM

Storage Class Code

10 - Combustible liquids

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