MDQ1

Imprint^® Methylated DNA Quantification Kit

To measure global DNA methylation shifts from as low as 10 ng DNA

• Synonym(s): DNA Methylation Quantification, DNA methylation shift
• NACRES: NA.54

Properties

• Quality Level: 200

Description

General description

Imprint™ Methylated DNA Quantification technology provides a rapid and reliable method to measure global DNA methylation shifts. The Imprint Methylated DNA Quantification Kit contains all reagents required to detect relative levels of methylated DNA. Up to 200 ng of purified DNA is bound to the wells of the assay strip. The methylated DNA is detected using the Capture and Detection antibodies, then quantified colorimetrically. The amount of methylated DNA present in the sample is proportional to the absorbance measured.

Application

Imprint^® Methylated DNA Quantification Kit has been used in global DNA methylation analysis.

Features and Benefits

• ELISA based format
• Procedure completes in 4 easy to follow steps
• All reagents supplied including control methylated DNA
• Detection limit is 5 ng of fully methylated DNA
• Input DNA may be as low as 10-200 ng
• Procedure completes in 3.5 hours
• 96 well format allows option of studying single samples or high-throughput studies
• Optimized antibody and reagents with high specificity to 5-mC; no cross-reactivity to unmethylated cytosine
• May be used with adherent and suspension cells, tissues, plasma and other body fluids such as saliva, urine and serum

Legal Information

Imprint is a registered trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

• M8570 Methylated Control DNA, 50 ng/μL

Safety Information

• Pictograms: GHS05,GHS07
• Signal Word: Warning
• Hazard Statements: H290 - H317 - H412
• Precautionary Statements: P234 - P261 - P272 - P273 - P280 - P302 + P352
• Hazard Classifications: Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1
• Storage Class Code: 8A - Combustible, corrosive hazardous materials
• WGK: WGK 3