All Photos(4)

N4142

Sigma-Aldrich

Anti-Neurofilament 200 antibody produced in rabbit

enhanced validation

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):
AntiNeurofilament heavy chain, Nf200 Antibody, NF200 Antibody - Anti-Neurofilament 200 antibody produced in rabbit
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 200 kDa

species reactivity

wide range, bovine

packaging

antibody small pack of 25 μL

enhanced validation

independent ( Antibodies)
Learn more about Antibody Enhanced Validation

application(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:80 using animal cerebellum sections
microarray: suitable
western blot: suitable

conjugate

unconjugated

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... NEFH(4744)
mouse ... Nefh(380684)
rat ... Nefh(24587)

General description

Neurofilament 200, also known as neurofilament heavy polypeptide (NEFH), is encoded by the gene mapped to human chromosome 22q12.2. The encoded protein has a molecular mass of 200kDa and belongs to the group of type IV intermediate filaments which are key constituents of the neuronal cytoskeleton. NEFH contains C-terminal motifs involved in the formation of sidearm projections which cross-link the neurofilaments.

Specificity

Shows a wide species cross-reactivity. Reacts with neurofilaments in the central and peripheral nervous system and localizes the neurofilament polypeptide.

Immunogen

Neurofilament 200 from bovine spinal cord.

Application

Anti-neurofilament 200 antibody produced in rabbit has been used for immunolabeling.
Anti-neurofilament 200 antibody produced in rabbit has been used:
  • for detection of neurofilaments using immunohistological staining
  • in human astrocytoma cell line
  • in post-mortem brain sections
  • in human cremaster muscles
  • for detection of neurofilaments by immunofluorescence staining in lumbar spinal cord sections of mice

Biochem/physiol Actions

Neurofilament 200 plays a vital role in cytoarchitecture organization. Downregulated expression of the gene has been observed in schizophrenia patients. Loss of gene expression is associated with the development of prostate carcinoma. Mutation in the gene leads to sporadic amyotrophic lateral sclerosis (ALS).
Neurofilament 200 assembly during development is critical for normal functioning of neuronal cells. Mutations in gene encoding neurofilaments is associated with neurological disorder called the Charcot–Marie–Tooth.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at -20°C. For continuous use, the product may be stored at 2-8°C for up to one month. For extended storage, solution may be frozen in working aliquots at –20°C. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Control of neurulation by the nucleosome assembly protein-1-like 2.
Rogner UC, et al.
Nature Genetics, 25, 431-435 (2000)
Raoul Torero Ibad et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 31(14), 5495-5503 (2011-04-08)
Retinal ganglion cells (RGCs) are the projection neurons from the eye to the brain and their loss results in visual impairment in a number of diseases. Transcription factors with a homeodomain can translocate between cells and, in at least one...
Hydrogen Peroxide-Induced Neurotoxicity in Cultured Cortical Cells Grown in SerumFree
and Serum-Containing Media
Ricart KC and Fiszman ML.
Neurochemical Research, 26, 801-808 (2001)
In vivo magnetic resonance imaging of ferritin-based reporter visualizes native neuroblast migration
Iordanova B and Ahrens ET.
Neuroimage, 59, 1004-1012 (2012)
Morphology and innervation of the human cremaster muscle in relation to its function.
Kayalioglu G, et al.
The Anatomical Record, 291(7), 790-796 (2008)

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