Merck
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P1860

Sigma-Aldrich

Protease Inhibitor Cocktail

for use in tissue culture media, DMSO solution

Synonym(s):
protease inhibitor
EC Number:
MDL number:
NACRES:
NA.77

Quality Level

form

DMSO solution

shipped in

dry ice

storage temp.

−20°C

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Specificity

Inhibits serine, cysteine, aspartic proteases, and aminopeptidases

Packaging

1 mL in glass bottle

Application

After 48 hours in medium, add fresh inhibitors in fresh medium.

Components

Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A

Other Notes

Mixture of protease inhibitors with broad specificity for the inhibtion of serine, cysteine, aspartic and aminopeptidases. Contains aprotinin, bestatin, leupeptin, E-64, and pepstatin A. Contains no metal chelators

Caution

After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.

Quantity

Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.

Physical form

Solution in DMSO (D 2650, Hybri-Max).

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C

Certificate of Analysis

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Certificate of Origin

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SDS

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