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P3813

Sigma-Aldrich

Phosphate buffered saline

powder, pH 7.4, for preparing 1 L solutions

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Synonym(s):
PBS
MDL number:
eCl@ss:
32129211
NACRES:
NA.25

Quality Level

form

powder

pH

7.4

solubility

water: soluble

application(s)

diagnostic assay manufacturing

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This Item
P38135P3688P4244
pH

7.4

pH

7.4

pH

7.4

pH

7.2 (25 °C)

solubility

water: soluble

solubility

water: soluble

solubility

water: soluble

solubility

-

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Phosphate-buffered saline (PBS) stands as a cornerstone in biological research due to its versatility and essential properties. One primary attribute is its isotonicity, closely resembling the pH, osmolarity, and ion concentrations of the human body. This makes PBS non-toxic to cells, playing a pivotal role in cell biology procedures such as washing cells, transporting tissues, and diluting cells and reagents. These applications ensure the preservation of cell viability and the maintenance of a physiological environment.

The buffering capacity of PBS, maintained through a phosphate-phosphoric acid equilibrium, provides a stable pH environment crucial for optimal enzyme activity and various biological processes. This makes PBS ideal for preparing reagents, protein dialysis, and immunohistochemistry, ensuring consistent and reliable results in experiments. Beyond cell biology, PBS may also find applications in diverse areas, including the creation of custom ELISA buffers to ensure optimal antibody-antigen interactions. Additionally, PBS can serve as a running buffer for Protein A/G chromatography, facilitating antibody purification based on specific antigen binding. In hematological and biochemical procedures, PBS is widely used for washing cells and tissues.

In summary, PBS emerges as an indispensable tool in both biological and biochemical research, offering isotonicity, buffering capacity, simplicity, and stability. Its diverse applications in cell biology, reagent preparation, protein purification, and various biological assays underscore its significance in advancing scientific knowledge and understanding.

Application

It has been utilized for rinsing fixed tissues and creating a suitable buffer environment in the immunolocalization process(1)It has been employed as a diluent to assess the performance of a ELISA test kit(2)It has been applied as a buffer in a immunohistochemistry procedure(3)
Phosphate buffered saline has been used:
  • in the washing solutions during immunofluorescence procedure.
  • for the suspension of bacterial cells.
  • during the fixation and immunostaining procedures.
  • for rinsing fixed tissues and creating a suitable buffer environment in the immunolocalization process
  • as a diluent to assess the performance of an ELISA test kit
  • as a buffer in an immunohistochemistry procedure

Features and Benefits

  • High-quality product suitable for multiple research applications
  • Can be used as a buffer component in biological and biochemical applications
  • Highly soluble in water

Packaging

Foil pouches

Reconstitution

Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.01 M phosphate buffered saline (NaCl 0.138 M; KCl - 0.0027 M); pH 7.4, at 25 °C.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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