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P4244

Sigma-Aldrich

Phosphate buffered saline

Autoclaved, pH 7.2 (25 °C)

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Synonym(s):
PBS
MDL number:
eCl@ss:
32129211
NACRES:
NA.25

description

Sodium chloride, 150 mM, and sodium phosphate, 150 mM

form

liquid

pH

7.2 (25 °C)

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

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This Item
P3688P3744P5368
Sigma-Aldrich

Sigma-Aldrich

P5368

Phosphate buffered saline

Premium Grade
pH

7.2 (25 °C)

pH

7.4

pH

7.6 (25 °C)

pH

7.4

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

storage temp.

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

description

Sodium chloride, 150 mM, and sodium phosphate, 150 mM

description

-

description

-

description

-

General description

Phosphate buffered saline (PBS) is an isotonic standard buffer solution. PBS imitates the pH, ion concentrations, and osmolarity of the human body. It serves as an ideal buffer for maintaining the osmolarity and physiological pH for biomedical dilutions in cell culture and protein chemistry. PBS is used for standard enzymatic tests with active metal-organic framework (MOF) biocomposites. PBS is considered non-toxic to cells.

Application

Phosphate buffered saline has been used:
  • for washing bacterial cells after harvesting by centrifugation
  • for washing the peritoneal cavity for isolation of peritoneal macrophages
  • as a control and to dilute phytohemagglutinin (PHA) to prepare subcutaneous injections and study the immune response in birds.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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