Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. Alkaline phosphatase is also used to prevent DNA self ligation and for radiolabeling.
100 units in serum bottle
Package size based on DEA units
Alkaline phosphatase is a hydrolase enzyme that dephosphorylates molecules such as nucleotides, proteins and alkaloids.
One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 °C.
Lyophilized fromTris-HCl buffer, with magnesium chloride and zinc chloride, pH 8.0
Protein determined by biuret.