3.7 (vs air)
1.08 mmHg ( 100 °C)
267 °C (lit.)
138-143 °C (lit.)
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Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Sens. 1 - STOT SE 1 Oral
6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
230.0 °F - closed cup
110 °C - closed cup
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This product is soluble in ethanol at a concentration of 50 mg/mL, with heating. A clear red to brown solution is observed.
This is an antifade reagent that is used as a fluorescent stabilizer. Do not confuse the p-phenylenediamine with o-phenylenediamine, which is used as a peroxidase substrate in ELISA procedures.
J. Immunological Methods, 43, 349 (1981) describes a solution that will reduce the fading of fluorescent materials. The recipe for this solution is: "Add 10 mL of phosphate-buffered saline (0.01 M PO4, pH 7.4 in 0.15 M NaCl) containing 100 mg of p-phenylenediamine to 90 ml of glycerol. The final pH should be adjusted to approximately 8.0 with 0.5 M carbonate-bicarbonate buffer (pH 9.0)."
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