PA0100
Plant Cell Viability Assay Kit
Fluorescent staining system to highlight viable and non-viable cells
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| Size/SKU | Availability | Price |
|---|---|---|
1 kit | Check Cart for Availability | CHF 527.00 |
About This Item
NACRES:
NA.84
UNSPSC Code:
10171502
Quality Segment
storage temp.
−20°C
General description
The Plant Cell Viability Assay Kit is designed for the differential viability staining of plant cells. The kit employs a dual color fluorescent staining system to highlight viable and non-viable cells. This procedure has been used to stain intact plant tissue, callus tissue, cell suspension culture, and protoplasts.
Application
Viable cells are living cells with intact plasma membranes. These cells can be distinguished by the presence of intracellular esterase activity. This activity is assayed through the enzymatic hydrolysis of fluorescein diacetate or related compounds, such as carboxyfluorescein or calcein AM. These lipophilic compounds are membrane-permeable and non-fluorescent. In the plant cell, they are hydrolyzed to highly polar fluorescent compounds. Because of their polar nature, these compounds are unable to diffuse across the plasma membrane and are retained within viable cells, producing an intense green fluorescence within the cytoplasm. Fluorescein diacetate was found to be the optimal dye for staining viable plant cells. It does not photobleach as quickly as calcein AM, and produces much less background fluorescence than carboxyfluorescein diacetate in plant cells. Non-viable cells could be either living or dead. They can be distinguished from viable cells by their non-intact or damaged plasma membranes. Propidium iodide and related compounds, such as ethidium homodimers-1 and -III, can only enter cells with damaged membranes, whereupon they intercalate into double-stranded nucleic acids. This results in a bright red fluorescence in non-viable cells, particularly in the nucleus where the concentration of nucleic acids is highest. Propidium iodide is the optimal dye for staining non-viable plant cells. In plant cells it specifically labels nucleic acids, whereas ethidium homodimers-1 and -III both bind non-specifically to the plant cell wall.
Other Notes
The components of this kit must be protected from light and stored at -20 °C with desiccant. Propidium iodide is stable in aqueous solution. However, fluorescein diacetate will hydrolyze in aqueous solution. Dilutions of the dyes should be used within one day.
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This Item | |||
|---|---|---|---|
| description Fluorescent staining system to highlight viable and non-viable cells | description for cellular ATP determination | description Live-dead cell viability kit for 3D and 2D cell cultures. Indicates live, dead and total cell count. Sufficient for 5 x 24-well plates or 12 x 96-well plates. | description - |
| storage temp. −20°C | storage temp. −20°C | storage temp. - | storage temp. −20°C (−15°C to −25°C) |
| Quality Level 200 | Quality Level 200 | Quality Level - | Quality Level - |
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
188.6 °F
flash_point_c
87 °C
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