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Merck

R4526

Sigma-Aldrich

Reference Dye for Quantitative PCR

100 ×, solution

Synonym(s):

Reference dye for qPCR, ROX

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0.3 ML
CHF 27.20
5 ML
CHF 276.00

CHF 27.20

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About This Item

UNSPSC Code:
41106300
NACRES:
NA.55

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form

solution

Quality Level

300

usage

sufficient for ≥600 reactions

concentration

100 ×

technique(s)

PCR: suitable

color

red

solubility

water: soluble

storage temp.

2-8°C

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This Item
E0781230914SCT036
Reference Dye for Quantitative PCR 100 ×, solution

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R4526

Reference Dye for Quantitative PCR

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technique(s)

PCR: suitable

technique(s)

-

technique(s)

-

technique(s)

cell based assay: suitable

concentration

100 ×

concentration

-

concentration

-

concentration

-

color

red

color

-

color

white

color

-

form

solution

form

-

form

lyophilized solid

form

-

solubility

water: soluble

solubility

-

solubility

DMSO: soluble, acetonitrile: water (1:1): soluble

solubility

-

Quality Level

300

Quality Level

200

Quality Level

100

Quality Level

-

General description

Sigma′s Reference Dye for quantitative polymerase chain reaction (qPCR) is a proprietary dye for use with real-time PCR. It is used for the normalization of reaction data when using SYBR Green, molecular probes, or dual-labeled probe chemistries for real-time detection. The Reference Dye is supplied as a 100× solution with a maximum excitation and emission of 586 nm and 605 nm, respectively. Instrument settings for ROX reference dye are satisfactory for the measurement of Reference Dye for qPCR.

Application

Reference Dye for Quantitative PCR has been used:
  • in the preparation of mastermix for real time-quantitative polymerase chain reaction (RT-qPCR)[1][2]
  • as a component of the reaction mixture for detection of Clostridium difficile by quantitative polymerase chain reaction (qPCR)[3][4]
  • for analyzing the degree of cellular DNA contamination by qPCR[5]

Other Notes

Reference Dye for Quantitative PCR is forR&D use only. Not for drug, household, or other uses.

Pictograms

Exclamation markEnvironment
GHS07,GHS09

Signal Word

Warning

Hazard Statements

H315,H317,H319,H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000450231
0000450231
0000360620
0000353112
0000353112

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Partial deletion of rng (RNase G)-enhanced homoethanol fermentation of xylose by the non-transgenic Escherichia coli RM10
Manow R, et al.
Journal of Industrial Microbiology & Biotechnology, 39(7), 977-985 (2012)
Kevin Antoine Brown et al.
Infection control and hospital epidemiology, 39(8), 917-923 (2018-08-10)
Clostridium difficile spores play an important role in transmission and can survive in the environment for several months. Optimal methods for measuring environmental C. difficile are unknown. We sought to determine whether increased sample surface area improved detection of C.
Ryan Manow et al.
World journal of microbiology & biotechnology, 36(4), 59-59 (2020-04-03)
An endogenous homoethanol pathway (glucose/1.2 xylose => 2 pyruvate => 2 ethanol) was previously engineered in Escherichia coli SZ410 via eliminating acid-producing pathways and anaerobic expression of the pyruvate dehydrogenase complex (aceEF-lpd operon). This ethanologenic derivative was subsequently engineered through adaptive evolution and partial
Araceli Melendez-Avalos et al.
Folia microbiologica, 65(1), 133-142 (2019-05-20)
This study aimed to analyze the proinflammatory cytokine mRNA expression in the urinary tract of BALB/c mice infected with bacterial strains with uropathogenic potential. Groups of four 6-week-old female BALB/c mice were intraurethrally inoculated with 5 × 107 colony-forming units (CFU) of
Gustav Johansson et al.
Molecular cancer therapeutics, 20(12), 2568-2576 (2021-09-24)
The majority of patients diagnosed with advanced gastrointestinal stromal tumors (GISTs) are successfully treated with a combination of surgery and tyrosine kinase inhibitors (TKIs). However, it remains challenging to monitor treatment efficacy and identify relapse early. Here, we utilized a
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