All Photos(2)

RAB0327

Sigma-Aldrich

Human Insulin ELISA Kit

for serum, plasma, cell culture supernatants and urine

NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

application(s)

ELISA: suitable
capture ELISA: suitable

input

sample type serum
sample type cell culture supernatant(s)
sample type urine
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 4 μIU/mL
standard curve range: 4.69-300 μIU/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... INS(3630)

General description

The Human Insulin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Insulin and Proinsulin in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human Insulin

Application

Human Insulin ELISA Kit has been used:
  • to measure the blood levels of human insulin and proinsulin in the transgenic mice
  • to measure serum insulin indaibetic rats
  • to measure insulin in Multiple Sclerosis patients

For research use only. Not for use in diagnostic procedures.

Human Insulin ELISA Kit has been used to determine human insulin and pro-insulin concentration in serum and HAMECs (human adipose microvascular endothelial cells) which were isolated from human visceral fat of nondiabetic patients.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Insulin is responsible for two types of actions, excitatory and inhibitory. In its excitatory role, it increases the uptake of glucose and lipid synthesis, and in its inhibitory role it inhibits glycogenolysis, gluconeogenesis, lipolysis, proteolysis and ketogenesis. Insulin plays a vital role in regulation of blood glucose level within the normal range. Abnormal insulin secretion leads to various disorders such as diabetes, hyperglycemia or hypoglycemia. Type I diabetes is a result of autoimmune destruction of β cells of pancreas, which leads to depletion of insulin. Mutant INS-gene Induced Diabetes of Youth (MIDY) syndrome is an autosomal dominant disorder caused by missense mutations, which lead to aberrant proinsulin folding. Impaired glucose tolerance (IGT) or non-insulin-dependent diabetes mellitus (NIDDM) is caused by resistance to insulin-stimulated glucose uptake.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)

  • RABSTOP3ELISA Stop Solution (Item I)

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  • RABWASH420X Wash Buffer (Item B)

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK Germany

WGK 3

Certificate of Analysis

Certificate of Origin

Production of recombinant human proinsulin in the milk of transgenic mice.
Qian X
Scientific Reports, 4:6465 (2014)
The Interplay between Inflammation and Metabolic Disturbances in Multiple Sclerosis
El-Malkey NF, et al.
Life Science Journal (2016)
Xi Qian et al.
Scientific reports, 4, 6465-6465 (2014-10-01)
There is a steady increasing demand for insulin worldwide. Current insulin manufacturing capacities can barely meet this increasing demand. The purpose of this study was to test the feasibility of producing human proinsulin in the milk of transgenic animals. Four...
Production of recombinant human proinsulin in the milk of transgenic mice
Qian X, et al..
Scientific Reports, 4, 6465-6465 (2014)
Insulin: understanding its action in health and disease.
P Sonksen et al.
British journal of anaesthesia, 85(1), 69-79 (2000-08-06)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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