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Rat Ins1 / Insulin ELISA Kit


species reactivity



kit of 96 wells (12 strips x 8 wells)


ELISA: suitable


sample type cell culture supernatant(s)
sample type plasma
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 5 μIU/mL
standard curve range: 4.69–300 μIU/mL

detection method


shipped in

wet ice

storage temp.


Gene Information

rat ... Ins1(24505)

General description

The antibody pair provided in this kit recognizes Rat Insulin.


For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Rat Ins1 / Insulin ELISA Kit has been used to measure serum insulin concentration.

Biochem/physiol Actions

Certain organisms such as mice, rat and xenopus possess two non-allelic insulin genes. These genes are Ins1 and Ins2, which are both functional. They encode insulin that is produced in a 1:2 ratio, respectively in the pancreas. The two genes are differentially expressed in mouse. The Ins1 gene maps to mouse chromosome 19 in the telomeric region. The insulin that is synthesized in the pancreas is crucial in maintaining glucose homeostasis. Loss of Ins-1 and Ins-2 expression leads to insulin deficient mice with diabetes mellitus, ketoacidosis, liver steatosis and retarded growth.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  • RABSTOP3ELISA Stop Solution (Item I)

  • RABWASH420X Wash Buffer (Item B)






Hazard Classifications

Met. Corr. 1

Storage Class Code

8B - Non-combustible, corrosive hazardous materials

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificate of Analysis

Certificate of Origin

Amanda C Okolie et al.
Bioscience reports, 39(2) (2017-08-10)
Recent studies have shown that Type 2 diabetes (T2D) in rats can result through a synergy that links obesity to insulin resistance and β-cell dysfunction. The present study achieved T2D via high fructose (20%w/v, p.o.), streptozotocin single dose (40 mg/kg...
Jingjing Li et al.
Neural plasticity, 2019, 7920540-7920540 (2019-03-27)
We investigated the effects of aerobic exercise on the expression of hippocampal synaptic plasticity-associated proteins in rats with type 2 diabetes and their possible mechanisms. A type 2 diabetes rat model was established with 8 weeks of high-fat diet combined...
Simone de Oliveira et al.
Obesity (Silver Spring, Md.), 29(1), 116-124 (2020-11-07)
The purpose of this study was to evaluate the effects of zinc (Zn) supplementation on metabolic and neuroinflammatory parameters in cafeteria diet (CAF)-induced obesity in Wistar rats. Animals were divided into four groups: control diet (CT); CT+Zn; CAF; CAF+Zn. The...
Stella Maris Honoré et al.
Journal of obesity, 2018, 5341384-5341384 (2018-12-05)
Smallanthus sonchifolius (yacon), a native plant of South America, was observed to improve lipid profile in rodents and humans. This study aimed to investigate the antiobesity properties of yacon roots in a high-fat-diet (HFD) model and the underlying mechanisms. A...
Tian Shen et al.
Experimental and therapeutic medicine, 16(4), 3121-3128 (2018-09-15)
Non-alcoholic fatty liver disease (NAFLD) is the most common liver disease. It is asymptomatic at presentation and is frequently identified among individuals with metabolic dysfunction, including obesity and diabetes. NAFLD is primarily characterized by the accumulation of triacylglycerol in the...

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