Sigma′s GenElute™ Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, plasma, serum, bacteria, yeast, fungi, plants and viruses. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Isolate true total RNA including siRNA and microRNA
Rapidly remove contaminating genomic DNA without the use of enzymes
No phenol or chloroform extractions
Purify high-quality RNA in 20 minutes
Extract RNA from as little as a single cell
Isolate from a wide variety of specimens
GenElute™ Total RNA Purification Kit is used for the extraction of total RNA:
From whole blood samples in the detection of Hepatitis C Virus using a reverse transcriptase-polymerase spiral reaction (RT-PSR) system.
From an overnight culture of bacterial strains in TSB medium in determining the effects of curcumin and silymarin on virulence and carbapenemase genes among multidrug-resistant Escherichia coli clinical isolates.
GenElute™ Total RNA Purification Kit has been used to:
Quantitative, real-time RT-PCR for both large mRNA and small RNA including miRNA
RT-PCR for both large mRNA and small RNA including miRNA
Next Generation Sequencing for RNA and miRNA
miRNA from plasma for discovery
microRNA cloning and amplification
PCR-based virus detection
PCR-based viable bacteria detection
Isolate total RNA of rat kidney tissues
Features and Benefits
No phenol: chloroform extractions - Total RNA is isolated without the use of harmful chemicals such as phenol or chloroform
Isolate total RNA from very small samples - Total RNA has been isolated and detected from as little as a single animal cell
Extremely efficient isolation of low abundance microRNA - GenElute′s Total RNA Purification Kit has been shown to be extremely efficient at recovering low abundance microRNA from plasma samples
Isolate a diversity of RNA species - All RNA species can be isolated, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA)
Fast and easy processing - Rapid spin-column format allows for the processing of 10 samples in 20 minutes
Isolate total RNA from a broad input source - Total RNA has been isolated from cultured animal cells, small tissue samples, LCM samples, bacteria, yeast, fungi, plants, viruses and various bodily fluid including blood, plasma, serum, saliva, nasal or throat swabs
No need for carrier RNA - Isolate all sizes of RNA without the use of carrier RNA
Multiple kit sizes available - This kit is available in both 50 prep and 100 prep sizes
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
GenElute is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
8A - Combustible, corrosive hazardous materials
Certificate of Analysis
Certificate of Origin
Rapid and Sensitive Detection of Hepatitis C Virus in Clinical Blood Samples Using Reverse Transcriptase Polymerase Spiral Reaction
Sun W, et al.
Journal of microbiology and biotechnology, 30(3), 459-468 (2020)
Silymarin and curcumin effects on virulence and carbapenemase genes among multidrug-resistant Escherichia coli clinical isolates
Eslami M, et al.
Revista da Associacao Medica Brasileira (1992), 29(4) (2018)
Glial reactivity in the dorsal horn of the spinal cord is a hallmark in most chronic pain conditions. Neuroinflammation-associated reactive glia, in particular astrocytes, have been shown to exhibit reduced mitochondrial respiratory function. Here, we studied the mitochondrial function at
Long non-coding RNAs (lncRNAs) play an important role in gene regulation. Several lncRNAs have been demonstrated to be associated with the diagnosis and prognosis of non-small cell lung cancer (NSCLC). The present study aimed to investigate the role of lncRNA
Journal of microbiology and biotechnology, 30(3), 459-468 (2020-01-03)
This study established a new polymerase spiral reaction (PSR) that combines with reverse transcription reactions for HCV detection targeting 5'UTR gene. To avoid cross-contamination of aerosols, an isothermal amplification tube (IAT), as a separate containment control, was used to judge
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