All Photos(3)

RNB200

Sigma-Aldrich

GenElute Total RNA Purification Maxi Kit

greener alternative
NACRES:
NA.52

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative category

Aligned

shipped in

ambient

storage temp.

room temp

General description

Sigma′s GenElute Total RNA Purification Maxi Kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants and viruses. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

  • Isolate true total RNA including siRNA and microRNA
  • No phenol extractions
  • Purify high-quality RNA in 40 minutes
  • Isolate total RNA from a wide variety of sources

Application

  • Quantitative, real-time RT-PCR for both large mRNA and small RNA including miRNA
  • RT-PCR for both large mRNA and small RNA including miRNA
  • Expression profiling
  • Next Generation Sequencing for RNA and miRNA
  • miRNA from plasma for discovery
  • microRNA cloning and amplification
  • PCR-based virus detection
  • PCR-based viable bacteria detection
  • Northern blotting
  • RNase protection
  • Primer extension
  • miRNA array
  • RNA-Seq
  • Nanostring
  • Fluidigm
  • Droplet/Digital PCR

Features and Benefits

  • Isolate a diversity of RNA species - All RNA species can be isolated, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA)
  • Fast and easy processing - Rapid spin-column format allows for the processing of 4 samples in under 40 minutes.
  • No phenol:chloroform extractions – Total RNA is isolated without the use of harmful chemicals such as phenol or chloroform.
  • Isolate total RNA from a wide variety of sources – Total RNA can be isolated from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants and viruses using this kit.
  • Isolate inhibitor-free RNA suitable for downstream applications - Purified RNA can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array analysis requiring the use of intact RNA
  • Isolate a diversity of RNA species - All RNA species can be isolated, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA)
  • Fast and easy processing - Rapid spin-column format allows for the processing of 4 samples in under 40 minutes.
  • No phenol:chloroform extractions – Total RNA is isolated without the use of harmful chemicals such as phenol or chloroform.
  • Isolate total RNA from a wide variety of sources – Total RNA can be isolated from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants and viruses using this kit.
  • Isolate inhibitor-free RNA suitable for downstream applications - Purified RNA can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array analysis requiring the use of intact RNA

Unit Definition

Preps

Other Notes

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1C

Supplementary Hazards

Storage Class Code

8A - Combustible, corrosive hazardous materials

Certificate of Analysis

Certificate of Origin

Jieru E Lin et al.
Cancer research, 76(2), 339-346 (2016-01-17)
Obesity is a well-known risk factor for colorectal cancer but precisely how it influences risks of malignancy remains unclear. During colon cancer development in humans or animals, attenuation of the colonic cell surface receptor guanylyl cyclase C (GUCY2C) that occurs

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service