Sigma′s GenElute™ Plasma/Serum RNA Purification Mini Kit provides a fast, reliable, reproducible and simple procedure for isolating circulating RNA and exosomal RNA from small plasma/serum inputs ranging from 10 μL to 25 μL, with various kit formats addressing different plasma/serum input volumes. Purification is based on spin column chromatography that uses Sigma′s proprietary resin separation matrix. The kit is designed to isolate all sizes of circulating RNA, including microRNA, as well as all sizes of exosomal RNA.
Sigma′s GenElute™ Plasma/Serum RNA Purification Mini Kit provides a clear advantage over other available kits in that they do not require phenol/chloroform or any protease treatments. RNA can be isolated from either fresh or frozen samples using these kits. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 10 μL to 100 μL. Typical yields of free-circulating and exosomal RNA vary depending on the input sample, as the amount of RNA present in plasma and serum will depend upon the health status of the individual.
Isolate all sizes of circulating and exosomal RNA, including microRNA
Versatile plasma/serum input ranging from 50 μL to 200 μL
No phenol extractions
No carrier RNA
Concentrate circulating RNA and Exosomal RNA into a flexible elution volume ranging from 10 μL to 25 μL
Purify high-quality RNA in 15-20 minutes
Reverse transcription qPCR
Reverse transcription PCR
RNase protection and primer extension
Expression array assays.
GenElute™ Plasma/Serum RNA Purification Mini Kit has been used for extraction of total serum RNA from human hepatocellular carcinoma (HCC) and colorectal cancer (CRC) samples.
Cancer cell international, 18, 116-116 (2018-08-21)
Accumulating data indicated that circRNA plays important roles in regulating many biological processes of the tumor, the present study is designated for exploring roles of the circ-ZEB1.33-miR-200a-3p-CDK6 regulating axis in human hepatocellular carcinoma (HCC). The regulation axis as predicted by
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