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RNB500

Sigma-Aldrich

GenElute Plasma/Serum RNA Purification Mini Kit

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General description

Sigma′s GenElute Plasma/Serum RNA Purification Mini Kit provides a fast, reliable, reproducible and simple procedure for isolating circulating RNA and exosomal RNA from small plasma/serum inputs ranging from 10 μL to 25 μL, with various kit formats addressing different plasma/serum input volumes. Purification is based on spin column chromatography that uses Sigma′s proprietary resin separation matrix. The kit is designed to isolate all sizes of circulating RNA, including microRNA, as well as all sizes of exosomal RNA.

Sigma′s GenElute Plasma/Serum RNA Purification Mini Kit provides a clear advantage over other available kits in that they do not require phenol/chloroform or any protease treatments. RNA can be isolated from either fresh or frozen samples using these kits. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 10 μL to 100 μL. Typical yields of free-circulating and exosomal RNA vary depending on the input sample, as the amount of RNA present in plasma and serum will depend upon the health status of the individual.

  • Isolate all sizes of circulating and exosomal RNA, including microRNA
  • Versatile plasma/serum input ranging from 50 μL to 200 μL
  • No phenol extractions
  • No carrier RNA
  • Concentrate circulating RNA and Exosomal RNA into a flexible elution volume ranging from 10 μL to 25 μL
  • Purify high-quality RNA in 15-20 minutes

Application

  • Reverse transcription qPCR
  • Reverse transcription PCR
  • Northern blotting
  • RNase protection and primer extension
  • Expression array assays.
  • miRNA array
  • RNA-Seq
  • Nanostring
  • Fluidigm
  • Droplet/Digital PCR

Biochem/physiol Actions

GenElute Plasma/Serum RNA Purification Mini Kit has been used for extraction of total serum RNA from human hepatocellular carcinoma (HCC) and colorectal cancer (CRC) samples.

Unit Definition

Preps

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

Certificate of Analysis

Certificate of Origin

Circ-ZEB1. 33 promotes the proliferation of human HCC by sponging miR-200a-3p and upregulating CDK6
Gong Y, et al.
Cancer Cell International, 18(1), 116-116 (2018)
MicroRNA-144 mediates chronic inflammation and tumorigenesis in colorectal cancer progression via regulating C-X-C motif chemokine ligand 11
Han B, et al.
Experimental and Therapeutic Medicine, 16(3), 1935-1943 (2018)
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Yuhua Gong et al.
Cancer cell international, 18, 116-116 (2018-08-21)
Accumulating data indicated that circRNA plays important roles in regulating many biological processes of the tumor, the present study is designated for exploring roles of the circ-ZEB1.33-miR-200a-3p-CDK6 regulating axis in human hepatocellular carcinoma (HCC). The regulation axis as predicted by

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