S0902

SSC Buffer

Name: SSC Buffer
Brand: SIGMA

for Northern and Southern blotting, powder blend

Synonym(s):

Saline-sodium citrate buffer

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About This Item

NACRES:

NA.25

UNSPSC Code:

41105319

Key Documents

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form

powder blend

pH

6.8-7.2 (20-25 °C, 263 mg/L in water)

solubility

water: 263 mg/mL, clear, colorless

foreign activity

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected

storage temp.

room temp

Quality Level

200

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Biochemicals

Biological Buffers

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1 of 4

This Item	85639	PPB020	11666681001
Sigma-Aldrich S0902 SSC Buffer	Sigma-Aldrich 85639 SSC Buffer	Sigma-Aldrich PPB020 SSC Buffer	Roche 11666681001 Buffers in a Box, Premixed SSC Buffer, 20x
Quality Level 200	Quality Level 100	Quality Level 100	Quality Level -
form powder blend	form powder blend	form powder	form solution
storage temp. room temp	storage temp. room temp	storage temp. -	storage temp. 20-25°C
pH 6.8-7.2 (20-25 °C, 263 mg/L in water)	pH -	pH 7.0	pH 7.0
solubility water: 263 mg/mL, clear, colorless	solubility H₂O: 0.263 g/mL, clear, colorless	solubility -	solubility -
foreign activity DNAse, none detected, Exonuclease, none detected, RNAse, none detected, Endonuclease, none detected, NICKase, none detected	foreign activity -	foreign activity -	foreign activity -

General description

SSC Buffer 20x Powder is a standard reagent in Southern and Northern blotting procedures. This concentrated powder should be reconstituted in molecular biology grade water to the indicated final volume before use (final solution is a 20x). The 20x concentrate can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.

Application

Saline-sodium citrate (SSC) Buffer has been used as a washing buffer in microarray technique.[1] It has also been used as a constituent of fluorescence in situ hybridization (FISH) buffer.[2]

Suitable for:

Preparing nucleic acids for hybridization
Hybridization buffer for Northern and Southern blots
Washing buffer for Northern and Southern blots

Features and Benefits

Multi-purpose use in hybridizations
Easily reconstituted and diluted to any concentration needed

Preparation Note

Final SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.

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Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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AUTEN-67, an autophagy-enhancing drug candidate with potent antiaging and neuroprotective effects

Papp D, et al.

Autophagy, 12(2), 273-286 (2016)

Protocol for the Preparation of Arabidopsis Meiotic Chromosome Spreads and Fluorescent in situ Hybridization

Bolanos V, et al.

The Plant Journal (2013)

Interaction of histone H1 with superhelical DNA. Sedimentation and electron microscopical studies at low salt concentration.

M Böttger et al.

Nucleic acids research, 9(20), 5253-5268 (1981-10-24)

Complexes of histones H1 with superhelical SV40 DNA obtained by direct mixing were studied in 0.1 SSC buffer corresponding to 0.02 M Na+. Depending on the molar input ratio H1/DNA three classes of sedimenting species were observed: (1) a component

A strand-specific burst in transcription of pericentric satellites is required for chromocenter formation and early mouse development.

Aline V Probst et al.

Developmental cell, 19(4), 625-638 (2010-10-19)

At the time of fertilization, the paternal genome lacks the typical configuration and marks characteristic of pericentric heterochromatin. It is thus essential to understand the dynamics of this region during early development, its importance during that time period and how

SUMOylation promotes de novo targeting of HP1α to pericentric heterochromatin.

Christèle Maison et al.

Nature genetics, 43(3), 220-227 (2011-02-15)

HP1 enrichment at pericentric heterochromatin is considered important for centromere function. Although HP1 binding to H3K9me3 can explain its accumulation at pericentric heterochromatin, how it is initially targeted there remains unclear. Here, in mouse cells, we reveal the presence of

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