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S4762

Sigma-Aldrich

Streptavidin from Streptomyces avidinii

essentially salt-free, lyophilized powder, ≥13 units/mg protein

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CAS Number:
MDL number:

form

essentially salt-free, lyophilized powder

Quality Level

specific activity

≥13 units/mg protein

mol wt

~60 kDa

capacity

4 mol/mol (Biotin)

application(s)

research pathology

storage temp.

−20°C

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This Item
85878S0677S2890
application(s)

research pathology

application(s)

-

application(s)

-

application(s)

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

specific activity

≥13 units/mg protein

specific activity

≥13 U/mg protein

specific activity

-

specific activity

≥700 DEA units/mg protein

mol wt

~60 kDa

mol wt

Mr ~60000

mol wt

-

mol wt

-

General description

Streptavidin derives its name from its bacterial source Streptomyces avidinii and from the hen egg-white protein, avidin, which has high affinity to biotin. Its homologous core shares 33% sequence similarity with avidin, as well as sharing a common tetrameric structure. It is a crystalline tetrameric protein, with a molecular weight of 4*15000Da. It binds four molecules of biotin. Streptavidin lacks carbohydrate and sulfur-containing amino acids.
Streptavidin is a crystalline protein isolated from the bacterium Streptomyces avidinii and possesses biotin-binding ability. It has four binding sites for biotin like avidin and binds four molecules of biotin. Streptavidin is now considered as a new type of biotin-binding protein.

Application

Streptavidin from Streptomyces avidinii has been used:
  • in wire functionalization
  • in streptavidin capture enzyme-linked immunosorbent assay (ELISA)
  • in microbubble preparation

Preparation Note

Affinity purified.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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A rapid streptavidin-capture ELISA specific for the detection of antibodies to feline foamy virus.
Winkler IG et al
Journal of Immunological Methods, 207(1), 69-77 (1997)
Mapping of functional domains in the p22(phox) subunit of flavocytochrome b(559) participating in the assembly of the NADPH oxidase complex by "peptide walking".
Dahan I et al
The Journal of Biological Chemistry, 277(10), 8421-8432 (2002)
Biotinylated CdSe/ZnSe nanocrystals for specific fluorescent labeling.
Charvet N et al
Journal of Materials Chemistry, 14.17 , 2638-2642 (2004)
Evaluation of a microwire sensor functionalized to detect Escherichia coli bacterial cells
Lu L and Jun S
Biosensors And Bioelectronics, 36(1), 257-261 (2012)
Viability of endothelial cells after ultrasound-mediated sonoporation: Influence of targeting, oscillation, and displacement of microbubbles
van R T, et al.
Journal of Controlled Release : Official Journal of the Controlled Release Society, 238, 197-211 (2016)

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