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SAE0063

Sigma-Aldrich

Alkaline Phosphatase Recombinant

solution (high-activity)

Synonym(s):
ALP
CAS Number:

recombinant

expressed in proprietary host

Quality Level

form

solution (high-activity)

shipped in

wet ice

storage temp.

2-8°C

General description

This high-activity recombinant alkaline phosphatase has a minimum Specific Activity of ≥ 15,000 U/mg (measured at 37 °C), and a minimum purity of ≥ 95% by HPLC.

No animal-derived components are used in the production process. This mitigates the risk of Bovine Spongiform Encephalopathy (BSE) and other animal-caused infections. This animal component free (ACF) recombinant alkaline phosphatase guarantees superior lot-to-lot consistency and eliminates BSE risk.

Application

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection.

Unit Definition

One unit hydrolyzes 1.0 μmole of p-nitrophenyl phosphate per minute at pH 9.8 (DEA) at 37 deg C.

Physical form

Solution in 3 M NaCl with triethanolamine, pH 7.5

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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